qPCR検出におけるODの選び方
Use stronger blocking when neighboring dye channels are close or detector gain is high. For routine qPCR, confirm both in-band transmission and out-of-band rejection before changing a standard filter set.
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一般的な qPCR 蛍光チャンネルの励起フィルター・蛍光フィルター・ダイクロイックミラーを比較。色素チャンネル・波長・FWHM・遮光レベル・機械的寸法を確認してから見積りをご依頼ください。
まず蛍光色素チャンネルと装置の光学系から始めます。励起・蛍光波長を合わせ、次に FWHM・OD 遮光・ダイクロイック遷移・入射角・フィルター寸法を確認します。マルチチャンネル qPCR システムでは、隣接色素間のスペクトル分離を十分に確保しクロストークを低減してください。
OPTOStokes PCR フィルターは、Bio-Rad CFX・Applied Biosystems QuantStudio・Roche LightCycler などリアルタイム蛍光定量 PCR 装置向けに設計されています。マルチチャンネル PCR システムで正確な蛍光検出を行うため、対象波長で高透過率を確保し、帯外遮光を深く確保しています。下記の蛍光色素―フィルター対応表で FAM・HEX・CY5・ROX などに適合するフィルターをすばやく特定できます。
| Fluorophore / Dye Family | Typical Excitation Filter | Typical Emission Filter | Typical Dichroic Mirror |
|---|---|---|---|
| ATTO 425 | BP430/24 | BP480/20 or BP480/30 | DM450 |
| FAM / FITC / SYBR Green | BP470/30, BP480/20, or BP485/20 | BP520/20 or BP525/20 | DM505 |
| HEX / VIC / JOE | BP520/20, BP525/20, or BP530/20 | BP565/22 or BP570/20 | DM550 |
| CY3 / TAMRA | BP543/20 | BP580/20 or BP585/20 | DM565 |
| ROX | BP565/22, BP570/20, BP580/20, or BP585/20 | BP615/40, BP612/20, or BP620/20 | DM590 or DM600 |
| ATTO 633 | BP620/20 | BP655/20 | DM635 |
| CY5 | BP624/40 or BP630/20 | BP694/40, BP675/20, or BP680/20 | DM647 |
| CY5.5 | BP675/20 or BP665/22* | BP725/30 or BP725/40 | DM700 |
| CY7 | BP715/70, BP725/30, or BP725/40 | BP800/30 or BP800/60 | DM770 |
* CY5.5 channel: BP665/22 vs BP675/20 requires internal verification before publication. See detailed filter sets table below for complete combinations.
| Model | 中心波長 (nm) | 半値全幅 (nm) | 透過率 | OD (In-band) | OD (Out-of-band) | Dimension |
|---|---|---|---|---|---|---|
| BP430/24 | 430±2 | 24±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP470/30 | 470±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP480/20 | 480±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP480/30 | 480±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP485/20 | 485±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP520/20 | 520±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP523/20 | 523±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP525/20 | 525±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP530/20 | 530±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP543/20 | 543±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP565/20 | 565±2 | 22±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP570/20 | 570±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP580/20 | 580±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP585/20 | 585±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP612/20 | 612±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP615/40 | 615±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP620/20 | 620±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP624/40 | 624±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP630/20 | 630±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP655/20 | 655±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP665/20 | 665±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP675/20 | 675±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP680/20 | 680±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP680/15 | 680±2 | 15±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP694/40 | 694±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP715/70 | 715±2 | 70±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP725/30 | 725±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP725/40 | 725±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP800/30 | 800±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP800/60 | 800±2 | 60±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| Model | AOI | 透過率 | Transmission Range (nm) | Reflectivity | Reflection Range (nm) |
|---|---|---|---|---|---|
| DM505 | 45-deg | Tave≥95% | 515-700 | R≥98% | 450-490 |
| DM550 | 45-deg | Tave≥95% | 560-700 | R≥98% | 450-540 |
| DM565 | 45-deg | Tave≥95% | 575-700 | R≥98% | 450-555 |
| DM590 | 45-deg | Tave≥95% | 603-700 | R≥98% | 450-580 |
| DM600 | 45-deg | Tave≥95% | 622-700 | R≥98% | 450-590 |
| DM635 | 45-deg | Tave≥95% | 650-700 | R≥98% | 450-630 |
| DM647 | 45-deg | Tave≥95% | 665-700 | R≥98% | 450-640 |
| DM700 | 45-deg | Tave≥95% | 715-800 | R≥98% | 450-685 |
| DM770 | 45-deg | Tave≥95% | 780-900 | R≥98% | 600-755 |
| DM500(SP) | 45-deg | Tave≥95% | 440-485 | R≥98% | 515-700 |
| DM550(SP) | 45-deg | Tave≥95% | 480-530 | R≥98% | 565-700 |
| DM565(SP) | 45-deg | Tave≥95% | 480-550 | R≥98% | 580-700 |
| DM600(SP) | 45-deg | Tave≥95% | 480-580 | R≥98% | 620-700 |
| DM650(SP) | 45-deg | Tave≥95% | 480-635 | R≥98% | 670-800 |
| DM690(SP) | 45-deg | Tave≥95% | 480-675 | R≥98% | 710-800 |
| DM450 | 45-deg | Tave≥95% | 465-680 | R≥98% | 380-445 |
| Fluorochrome | Excitation Filter (nm) | Emission Filter (nm) | Dichroic Mirror (nm) |
|---|---|---|---|
| Atto425 | BP430/24 | BP480/30 | DM450 |
| Atto425 | BP430/24 | BP480/20 | DM450 |
| FAM | BP470/30 | BP520/20 | DM505 |
| FAM | BP470/30 | BP525/20 | DM505 |
| FAM | BP480/20 | BP520/20 | DM505 |
| FAM | BP485/20 | BP525/20 | DM505 |
| HEX,VIC,JOE | BP520/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP525/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP530/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP530/20 | BP570/20 | DM550 |
| CY3,TAMRA | BP543/20 | BP580/20 | DM565 |
| CY3,TAMRA | BP543/20 | BP585/20 | DM565 |
| ROX | BP565/22 | BP615/40 | DM590 |
| ROX | BP570/20 | BP612/20 | DM590 |
| ROX | BP580/20 | BP620/20 | DM600 |
| ROX | BP585/20 | BP620/20 | DM600 |
| Atto 633 | BP620/20 | BP655/20 | DM635 |
| CY5 | BP624/40 | BP694/40 | DM647 |
| CY5 | BP624/40 | BP675/20 | DM647 |
| CY5 | BP630/20 | BP675/20 | DM647 |
| CY5 | BP630/20 | BP680/20 | DM647 |
| CY5.5 | BP675/20 | BP725/30 | DM700 |
| CY5.5 | BP665/22 | BP725/40 | DM700 |
| CY7 | BP715/70 | BP800/60 | DM770 |
| CY7 | BP725/30 | BP800/30 | DM770 |
| CY7 | BP725/40 | BP800/30 | DM770 |
Use stronger blocking when neighboring dye channels are close or detector gain is high. For routine qPCR, confirm both in-band transmission and out-of-band rejection before changing a standard filter set.
PCR instruments may use compact optical paths, fixed holders, and non-standard angles. Check clear aperture, thickness, and AOI before assuming a wavelength match is enough.
Custom CWL or FWHM is useful when an assay uses uncommon dyes, multiplex channels, or a legacy instrument with limited space.
The page supports common qPCR channels such as FAM, SYBR Green, HEX, VIC, JOE, ROX, CY5, CY5.5, and CY7.
Most fluorescence instruments use a matched optical set. The excitation filter selects the light source band, the dichroic directs the beam, and the emission filter passes the fluorescence signal to the detector.
Yes. Wavelength, bandwidth, OD blocking, size, thickness, and channel combinations can be adjusted for OEM optical layouts.
Quality & Compliance
For OEM programs, laboratory instruments, and industrial systems, OPTOStokes keeps quality, material compliance, and engineering support visible from the start of supplier evaluation.
Process control, inspection records, and repeatable coating workflows for production optical components.
Compliance documentation can be prepared for qualified projects and regulated supply chains.
Wavelength, FWHM, substrate, AOI, size, and mounting requirements can be reviewed with engineering.
Share target wavelength, blocking level, transmittance, and drawing requirements for a focused quote.
Our engineers will help you select the perfect optical solution.
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