Cómo elegir la OD para detección qPCR
Use stronger blocking when neighboring dye channels are close or detector gain is high. For routine qPCR, confirm both in-band transmission and out-of-band rejection before changing a standard filter set.
Filtros ópticos PCR para instrumentos qPCR
Compare filtros de excitación, emisión y dicroicos para canales qPCR comunes. Revise canales de colorante, longitudes de onda, FWHM, niveles de bloqueo y dimensiones mecánicas antes de solicitar cotización.
Más de 12 años de experiencia
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Certificación ISO 9001
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Envío a más de 60 países
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Tasa de calidad del 99,8%
Cómo elegir
Comience con el canal del colorante y el trayecto óptico del instrumento. Primero haga coincidir las longitudes de onda de excitación y emisión, luego confirme FWHM, bloqueo OD, transición dicroica, ángulo de incidencia y dimensiones del filtro. Para sistemas qPCR multicanal, mantenga suficiente separación espectral entre colorantes vecinos para reducir el crosstalk.
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Filtros PCR de fluorescencia
Los filtros PCR de OPTOStokes están diseñados para instrumentos de PCR cuantitativa en tiempo real, incluyendo Bio-Rad CFX, Applied Biosystems QuantStudio y Roche LightCycler. Estos filtros ofrecen alta transmitancia en las longitudes de onda objetivo con bloqueo profundo fuera de banda para una detección de fluorescencia precisa en sistemas PCR multicanal. Use la tabla de correspondencia fluoróforo-filtro para identificar rápidamente los filtros adecuados para FAM, HEX, CY5, ROX y más.
Fluorophore Channel Mapping
| Fluorophore / Dye Family | Typical Excitation Filter | Typical Emission Filter | Typical Dichroic Mirror |
|---|---|---|---|
| ATTO 425 | BP430/24 | BP480/20 or BP480/30 | DM450 |
| FAM / FITC / SYBR Green | BP470/30, BP480/20, or BP485/20 | BP520/20 or BP525/20 | DM505 |
| HEX / VIC / JOE | BP520/20, BP525/20, or BP530/20 | BP565/22 or BP570/20 | DM550 |
| CY3 / TAMRA | BP543/20 | BP580/20 or BP585/20 | DM565 |
| ROX | BP565/22, BP570/20, BP580/20, or BP585/20 | BP615/40, BP612/20, or BP620/20 | DM590 or DM600 |
| ATTO 633 | BP620/20 | BP655/20 | DM635 |
| CY5 | BP624/40 or BP630/20 | BP694/40, BP675/20, or BP680/20 | DM647 |
| CY5.5 | BP675/20 or BP665/22* | BP725/30 or BP725/40 | DM700 |
| CY7 | BP715/70, BP725/30, or BP725/40 | BP800/30 or BP800/60 | DM770 |
* CY5.5 channel: BP665/22 vs BP675/20 requires internal verification before publication. See detailed filter sets table below for complete combinations.
Fluorophore Channel Mapping
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| Model | Longitud de onda central (nm) | Ancho a mitad de altura (nm) | Transmitancia | OD (In-band) | OD (Out-of-band) | Dimension |
|---|---|---|---|---|---|---|
| BP430/24 | 430±2 | 24±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP470/30 | 470±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP480/20 | 480±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP480/30 | 480±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP485/20 | 485±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP520/20 | 520±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP523/20 | 523±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP525/20 | 525±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP530/20 | 530±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP543/20 | 543±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP565/20 | 565±2 | 22±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP570/20 | 570±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP580/20 | 580±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP585/20 | 585±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP612/20 | 612±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP615/40 | 615±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP620/20 | 620±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP624/40 | 624±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP630/20 | 630±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP655/20 | 655±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP665/20 | 665±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP675/20 | 675±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP680/20 | 680±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP680/15 | 680±2 | 15±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP694/40 | 694±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP715/70 | 715±2 | 70±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP725/30 | 725±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP725/40 | 725±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP800/30 | 800±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP800/60 | 800±2 | 60±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
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| Model | AOI | Transmitancia | Transmission Range (nm) | Reflectivity | Reflection Range (nm) |
|---|---|---|---|---|---|
| DM505 | 45-deg | Tave≥95% | 515-700 | R≥98% | 450-490 |
| DM550 | 45-deg | Tave≥95% | 560-700 | R≥98% | 450-540 |
| DM565 | 45-deg | Tave≥95% | 575-700 | R≥98% | 450-555 |
| DM590 | 45-deg | Tave≥95% | 603-700 | R≥98% | 450-580 |
| DM600 | 45-deg | Tave≥95% | 622-700 | R≥98% | 450-590 |
| DM635 | 45-deg | Tave≥95% | 650-700 | R≥98% | 450-630 |
| DM647 | 45-deg | Tave≥95% | 665-700 | R≥98% | 450-640 |
| DM700 | 45-deg | Tave≥95% | 715-800 | R≥98% | 450-685 |
| DM770 | 45-deg | Tave≥95% | 780-900 | R≥98% | 600-755 |
| DM500(SP) | 45-deg | Tave≥95% | 440-485 | R≥98% | 515-700 |
| DM550(SP) | 45-deg | Tave≥95% | 480-530 | R≥98% | 565-700 |
| DM565(SP) | 45-deg | Tave≥95% | 480-550 | R≥98% | 580-700 |
| DM600(SP) | 45-deg | Tave≥95% | 480-580 | R≥98% | 620-700 |
| DM650(SP) | 45-deg | Tave≥95% | 480-635 | R≥98% | 670-800 |
| DM690(SP) | 45-deg | Tave≥95% | 480-675 | R≥98% | 710-800 |
| DM450 | 45-deg | Tave≥95% | 465-680 | R≥98% | 380-445 |
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| Fluorochrome | Excitation Filter (nm) | Emission Filter (nm) | Dichroic Mirror (nm) |
|---|---|---|---|
| Atto425 | BP430/24 | BP480/30 | DM450 |
| Atto425 | BP430/24 | BP480/20 | DM450 |
| FAM | BP470/30 | BP520/20 | DM505 |
| FAM | BP470/30 | BP525/20 | DM505 |
| FAM | BP480/20 | BP520/20 | DM505 |
| FAM | BP485/20 | BP525/20 | DM505 |
| HEX,VIC,JOE | BP520/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP525/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP530/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP530/20 | BP570/20 | DM550 |
| CY3,TAMRA | BP543/20 | BP580/20 | DM565 |
| CY3,TAMRA | BP543/20 | BP585/20 | DM565 |
| ROX | BP565/22 | BP615/40 | DM590 |
| ROX | BP570/20 | BP612/20 | DM590 |
| ROX | BP580/20 | BP620/20 | DM600 |
| ROX | BP585/20 | BP620/20 | DM600 |
| Atto 633 | BP620/20 | BP655/20 | DM635 |
| CY5 | BP624/40 | BP694/40 | DM647 |
| CY5 | BP624/40 | BP675/20 | DM647 |
| CY5 | BP630/20 | BP675/20 | DM647 |
| CY5 | BP630/20 | BP680/20 | DM647 |
| CY5.5 | BP675/20 | BP725/30 | DM700 |
| CY5.5 | BP665/22 | BP725/40 | DM700 |
| CY7 | BP715/70 | BP800/60 | DM770 |
| CY7 | BP725/30 | BP800/30 | DM770 |
| CY7 | BP725/40 | BP800/30 | DM770 |
Notas técnicas relacionadas
Ajuste de filtros a la geometría del instrumento
PCR instruments may use compact optical paths, fixed holders, and non-standard angles. Check clear aperture, thickness, and AOI before assuming a wavelength match is enough.
Cuándo tienen sentido los filtros PCR personalizados
Custom CWL or FWHM is useful when an assay uses uncommon dyes, multiplex channels, or a legacy instrument with limited space.
Preguntas frecuentes
¿Qué colorantes PCR se suelen emparejar en esta página?
The page supports common qPCR channels such as FAM, SYBR Green, HEX, VIC, JOE, ROX, CY5, CY5.5, and CY7.
¿Necesito filtros de excitación, emisión y dicroicos en conjunto?
Most fluorescence instruments use a matched optical set. The excitation filter selects the light source band, the dichroic directs the beam, and the emission filter passes the fluorescence signal to the detector.
¿Se pueden personalizar estos filtros PCR para instrumentos OEM?
Yes. Wavelength, bandwidth, OD blocking, size, thickness, and channel combinations can be adjusted for OEM optical layouts.
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