איך לבחור OD לזיהוי qPCR
Use stronger blocking when neighboring dye channels are close or detector gain is high. For routine qPCR, confirm both in-band transmission and out-of-band rejection before changing a standard filter set.
מסננים אופטיים PCR למכשירי qPCR
השוו מסנני עירור, פליטה ודיכרואיים לערוצי qPCR נפוצים. בחנו ערוצי צבענים, אורכי גל, FWHM, רמות חסימה ומידות מכניות לפני בקשת הצעת מחיר.
ניסיון של 12+ שנים
|
תקן ISO 9001
|
משלוח ל-60+ מדינות
|
שיעור איכות 99.8%
איך לבחור
התחילו מערוץ הצבען והמסלול האופטי של המכשיר. התאימו תחילה אורכי גל עירור ופליטה, ולאחר מכן אשרו FWHM, חסימת OD, מעבר דיכרואי, זווית פגיעה ומידות המסנן. במערכות qPCR רב-ערוציות, שמרו על הפרדה ספקטרלית מספקת בין צבענים שכנים להפחתת crosstalk.
1
מסנני PCR פלואורסצנטיים
מסנני PCR של OPTOStokes מיועדים למכשירי PCR כמותי פלואורסצנטיים בזמן אמת, כולל Bio-Rad CFX, Applied Biosystems QuantStudio ו-Roche LightCycler. מסננים אלו מספקים העברה גבוהה באורכי הגל המבוקשים עם חסימה עמוקה מחוץ לפס לזיהוי פלואורסצנציה מדויק במערכות PCR רב-ערוציות. השתמשו בטבלת מיפוי פלואורופור-למסנן להלן לזיהוי מהיר של המסננים המתאימים עבור FAM, HEX, CY5, ROX ועוד.
Fluorophore Channel Mapping
| Fluorophore / Dye Family | Typical Excitation Filter | Typical Emission Filter | Typical Dichroic Mirror |
|---|---|---|---|
| ATTO 425 | BP430/24 | BP480/20 or BP480/30 | DM450 |
| FAM / FITC / SYBR Green | BP470/30, BP480/20, or BP485/20 | BP520/20 or BP525/20 | DM505 |
| HEX / VIC / JOE | BP520/20, BP525/20, or BP530/20 | BP565/22 or BP570/20 | DM550 |
| CY3 / TAMRA | BP543/20 | BP580/20 or BP585/20 | DM565 |
| ROX | BP565/22, BP570/20, BP580/20, or BP585/20 | BP615/40, BP612/20, or BP620/20 | DM590 or DM600 |
| ATTO 633 | BP620/20 | BP655/20 | DM635 |
| CY5 | BP624/40 or BP630/20 | BP694/40, BP675/20, or BP680/20 | DM647 |
| CY5.5 | BP675/20 or BP665/22* | BP725/30 or BP725/40 | DM700 |
| CY7 | BP715/70, BP725/30, or BP725/40 | BP800/30 or BP800/60 | DM770 |
* CY5.5 channel: BP665/22 vs BP675/20 requires internal verification before publication. See detailed filter sets table below for complete combinations.
Fluorophore Channel Mapping
–
Showing 0 of 0
| Model | אורך גל מרכזי (nm) | רוחב חצי מקסימום (nm) | העברה | OD (In-band) | OD (Out-of-band) | Dimension |
|---|---|---|---|---|---|---|
| BP430/24 | 430±2 | 24±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP470/30 | 470±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP480/20 | 480±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP480/30 | 480±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP485/20 | 485±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP520/20 | 520±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP523/20 | 523±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP525/20 | 525±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP530/20 | 530±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP543/20 | 543±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP565/20 | 565±2 | 22±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP570/20 | 570±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP580/20 | 580±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP585/20 | 585±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP612/20 | 612±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP615/40 | 615±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP620/20 | 620±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP624/40 | 624±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP630/20 | 630±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP655/20 | 655±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP665/20 | 665±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP675/20 | 675±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP680/20 | 680±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP680/15 | 680±2 | 15±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP694/40 | 694±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP715/70 | 715±2 | 70±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP725/30 | 725±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP725/40 | 725±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP800/30 | 800±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP800/60 | 800±2 | 60±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
Showing 0 of 0
| Model | AOI | העברה | Transmission Range (nm) | Reflectivity | Reflection Range (nm) |
|---|---|---|---|---|---|
| DM505 | 45-deg | Tave≥95% | 515-700 | R≥98% | 450-490 |
| DM550 | 45-deg | Tave≥95% | 560-700 | R≥98% | 450-540 |
| DM565 | 45-deg | Tave≥95% | 575-700 | R≥98% | 450-555 |
| DM590 | 45-deg | Tave≥95% | 603-700 | R≥98% | 450-580 |
| DM600 | 45-deg | Tave≥95% | 622-700 | R≥98% | 450-590 |
| DM635 | 45-deg | Tave≥95% | 650-700 | R≥98% | 450-630 |
| DM647 | 45-deg | Tave≥95% | 665-700 | R≥98% | 450-640 |
| DM700 | 45-deg | Tave≥95% | 715-800 | R≥98% | 450-685 |
| DM770 | 45-deg | Tave≥95% | 780-900 | R≥98% | 600-755 |
| DM500(SP) | 45-deg | Tave≥95% | 440-485 | R≥98% | 515-700 |
| DM550(SP) | 45-deg | Tave≥95% | 480-530 | R≥98% | 565-700 |
| DM565(SP) | 45-deg | Tave≥95% | 480-550 | R≥98% | 580-700 |
| DM600(SP) | 45-deg | Tave≥95% | 480-580 | R≥98% | 620-700 |
| DM650(SP) | 45-deg | Tave≥95% | 480-635 | R≥98% | 670-800 |
| DM690(SP) | 45-deg | Tave≥95% | 480-675 | R≥98% | 710-800 |
| DM450 | 45-deg | Tave≥95% | 465-680 | R≥98% | 380-445 |
Showing 0 of 0
| Fluorochrome | Excitation Filter (nm) | Emission Filter (nm) | Dichroic Mirror (nm) |
|---|---|---|---|
| Atto425 | BP430/24 | BP480/30 | DM450 |
| Atto425 | BP430/24 | BP480/20 | DM450 |
| FAM | BP470/30 | BP520/20 | DM505 |
| FAM | BP470/30 | BP525/20 | DM505 |
| FAM | BP480/20 | BP520/20 | DM505 |
| FAM | BP485/20 | BP525/20 | DM505 |
| HEX,VIC,JOE | BP520/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP525/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP530/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP530/20 | BP570/20 | DM550 |
| CY3,TAMRA | BP543/20 | BP580/20 | DM565 |
| CY3,TAMRA | BP543/20 | BP585/20 | DM565 |
| ROX | BP565/22 | BP615/40 | DM590 |
| ROX | BP570/20 | BP612/20 | DM590 |
| ROX | BP580/20 | BP620/20 | DM600 |
| ROX | BP585/20 | BP620/20 | DM600 |
| Atto 633 | BP620/20 | BP655/20 | DM635 |
| CY5 | BP624/40 | BP694/40 | DM647 |
| CY5 | BP624/40 | BP675/20 | DM647 |
| CY5 | BP630/20 | BP675/20 | DM647 |
| CY5 | BP630/20 | BP680/20 | DM647 |
| CY5.5 | BP675/20 | BP725/30 | DM700 |
| CY5.5 | BP665/22 | BP725/40 | DM700 |
| CY7 | BP715/70 | BP800/60 | DM770 |
| CY7 | BP725/30 | BP800/30 | DM770 |
| CY7 | BP725/40 | BP800/30 | DM770 |
הערות טכניות קשורות
התאמת מסננים לגאומטריית המכשיר
PCR instruments may use compact optical paths, fixed holders, and non-standard angles. Check clear aperture, thickness, and AOI before assuming a wavelength match is enough.
מתי מסנני PCR מותאמים אישית הם הפתרון הנכון
Custom CWL or FWHM is useful when an assay uses uncommon dyes, multiplex channels, or a legacy instrument with limited space.
שאלות נפוצות
אילו צבעני PCR נפוצים מותאמים בעמוד זה?
The page supports common qPCR channels such as FAM, SYBR Green, HEX, VIC, JOE, ROX, CY5, CY5.5, and CY7.
האם נדרשים מסנני עירור, פליטה ודיכרואיים יחד?
Most fluorescence instruments use a matched optical set. The excitation filter selects the light source band, the dichroic directs the beam, and the emission filter passes the fluorescence signal to the detector.
האם ניתן להתאים מסנני PCR אלה למכשירי OEM?
Yes. Wavelength, bandwidth, OD blocking, size, thickness, and channel combinations can be adjusted for OEM optical layouts.
Quality & Compliance
Documentation-ready optical filter supply
For OEM programs, laboratory instruments, and industrial systems, OPTOStokes keeps quality, material compliance, and engineering support visible from the start of supplier evaluation.
Quality system
ISO 9001 managed production
Process control, inspection records, and repeatable coating workflows for production optical components.
Material compliance
RoHS / REACH support
Compliance documentation can be prepared for qualified projects and regulated supply chains.
Factory-direct
Custom coating and dimensions
Wavelength, FWHM, substrate, AOI, size, and mounting requirements can be reviewed with engineering.
Procurement ready
Quote and data review
Share target wavelength, blocking level, transmittance, and drawing requirements for a focused quote.
Ready to Find Your Filter?
Our engineers will help you select the perfect optical solution.
Product Preview
Product Comparison
| Specification |
|---|