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Bộ lọc quang học PCR cho thiết bị qPCR

So sánh excitation filter・emission filter・dichroic mirror cho các kênh huỳnh quang qPCR phổ biến. Xem kênh fluorophore・bước sóng・FWHM・mức chặn sáng・kích thước cơ khí trước khi yêu cầu báo giá.

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Optical Filter Catalog › Bộ lọc PCR huỳnh quang

Mã sản phẩm

Hướng dẫn lựa chọn

Bắt đầu với kênh fluorophore và hệ quang của thiết bị. Ghép bước sóng excitation và emission, sau đó kiểm tra FWHM・chặn OD・chuyển tiếp dichroic・góc tới・kích thước bộ lọc. Với hệ thống qPCR đa kênh, đảm bảo đủ khoảng cách phổ giữa các fluorophore lân cận để giảm nhiễu xuyên kênh.

1

Bộ lọc PCR huỳnh quang

Bộ lọc PCR OPTOStokes được thiết kế cho các thiết bị real-time PCR như Bio-Rad CFX・Applied Biosystems QuantStudio・Roche LightCycler. Đảm bảo độ truyền qua cao tại bước sóng mục tiêu và chặn sáng sâu ngoài dải để phát hiện huỳnh quang chính xác trong hệ thống PCR đa kênh. Bảng đối chiếu fluorophore-bộ lọc dưới đây giúp nhanh chóng xác định bộ lọc phù hợp cho FAM・HEX・CY5・ROX.

View full PCR Optical Filters

Fluorophore Channel Mapping

Quick Reference: Fluorophore to Filter Mapping for qPCR
Fluorophore / Dye Family Typical Excitation Filter Typical Emission Filter Typical Dichroic Mirror
ATTO 425BP430/24BP480/20 or BP480/30DM450
FAM / FITC / SYBR GreenBP470/30, BP480/20, or BP485/20BP520/20 or BP525/20DM505
HEX / VIC / JOEBP520/20, BP525/20, or BP530/20BP565/22 or BP570/20DM550
CY3 / TAMRABP543/20BP580/20 or BP585/20DM565
ROXBP565/22, BP570/20, BP580/20, or BP585/20BP615/40, BP612/20, or BP620/20DM590 or DM600
ATTO 633BP620/20BP655/20DM635
CY5BP624/40 or BP630/20BP694/40, BP675/20, or BP680/20DM647
CY5.5BP675/20 or BP665/22*BP725/30 or BP725/40DM700
CY7BP715/70, BP725/30, or BP725/40BP800/30 or BP800/60DM770

* CY5.5 channel: BP665/22 vs BP675/20 requires internal verification before publication. See detailed filter sets table below for complete combinations.

Fluorophore Channel Mapping

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Model Bước sóng trung tâm (nm) FWHM (nm) Độ truyền qua OD (In-band) OD (Out-of-band) Dimension
BP430/24 430±2 24±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP470/30 470±2 30±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP480/20 480±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP480/30 480±2 30±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP485/20 485±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP520/20 520±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP523/20 523±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP525/20 525±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP530/20 530±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP543/20 543±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP565/20 565±2 22±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP570/20 570±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP580/20 580±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP585/20 585±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP612/20 612±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP615/40 615±2 40±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP620/20 620±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP624/40 624±2 40±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP630/20 630±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP655/20 655±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP665/20 665±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP675/20 675±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP680/20 680±2 20±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP680/15 680±2 15±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP694/40 694±2 40±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP715/70 715±2 70±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP725/30 725±2 30±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP725/40 725±2 40±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP800/30 800±2 30±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
BP800/60 800±2 60±2 Tave≥90% ODave>6@350-800 OD>3@800-1050 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm
ass="sub-title">PCR Dichroic Mirrors
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Model AOI Độ truyền qua Transmission Range (nm) Reflectivity Reflection Range (nm)
DM505 45-deg Tave≥95% 515-700 R≥98% 450-490
DM550 45-deg Tave≥95% 560-700 R≥98% 450-540
DM565 45-deg Tave≥95% 575-700 R≥98% 450-555
DM590 45-deg Tave≥95% 603-700 R≥98% 450-580
DM600 45-deg Tave≥95% 622-700 R≥98% 450-590
DM635 45-deg Tave≥95% 650-700 R≥98% 450-630
DM647 45-deg Tave≥95% 665-700 R≥98% 450-640
DM700 45-deg Tave≥95% 715-800 R≥98% 450-685
DM770 45-deg Tave≥95% 780-900 R≥98% 600-755
DM500(SP) 45-deg Tave≥95% 440-485 R≥98% 515-700
DM550(SP) 45-deg Tave≥95% 480-530 R≥98% 565-700
DM565(SP) 45-deg Tave≥95% 480-550 R≥98% 580-700
DM600(SP) 45-deg Tave≥95% 480-580 R≥98% 620-700
DM650(SP) 45-deg Tave≥95% 480-635 R≥98% 670-800
DM690(SP) 45-deg Tave≥95% 480-675 R≥98% 710-800
DM450 45-deg Tave≥95% 465-680 R≥98% 380-445
ass="sub-title">Typical PCR Filter Sets
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Fluorochrome Excitation Filter (nm) Emission Filter (nm) Dichroic Mirror (nm)
Atto425BP430/24BP480/30DM450
Atto425BP430/24BP480/20DM450
FAMBP470/30BP520/20DM505
FAMBP470/30BP525/20DM505
FAMBP480/20BP520/20DM505
FAMBP485/20BP525/20DM505
HEX,VIC,JOEBP520/20BP565/22DM550
HEX,VIC,JOEBP525/20BP565/22DM550
HEX,VIC,JOEBP530/20BP565/22DM550
HEX,VIC,JOEBP530/20BP570/20DM550
CY3,TAMRABP543/20BP580/20DM565
CY3,TAMRABP543/20BP585/20DM565
ROXBP565/22BP615/40DM590
ROXBP570/20BP612/20DM590
ROXBP580/20BP620/20DM600
ROXBP585/20BP620/20DM600
Atto 633BP620/20BP655/20DM635
CY5BP624/40BP694/40DM647
CY5BP624/40BP675/20DM647
CY5BP630/20BP675/20DM647
CY5BP630/20BP680/20DM647
CY5.5BP675/20BP725/30DM700
CY5.5BP665/22BP725/40DM700
CY7BP715/70BP800/60DM770
CY7BP725/30BP800/30DM770
CY7BP725/40BP800/30DM770

Danh mục liên quan

Tài liệu kỹ thuật liên quan

Cách chọn OD cho phát hiện qPCR

Use stronger blocking when neighboring dye channels are close or detector gain is high. For routine qPCR, confirm both in-band transmission and out-of-band rejection before changing a standard filter set.

Ghép bộ lọc với hệ quang thiết bị

PCR instruments may use compact optical paths, fixed holders, and non-standard angles. Check clear aperture, thickness, and AOI before assuming a wavelength match is enough.

Trường hợp bộ lọc PCR tùy chỉnh hiệu quả

Custom CWL or FWHM is useful when an assay uses uncommon dyes, multiplex channels, or a legacy instrument with limited space.

Câu hỏi thường gặp

Trang này hỗ trợ những fluorophore PCR nào?

The page supports common qPCR channels such as FAM, SYBR Green, HEX, VIC, JOE, ROX, CY5, CY5.5, and CY7.

Có cần bộ excitation・emission・dichroic đầy đủ không?

Most fluorescence instruments use a matched optical set. The excitation filter selects the light source band, the dichroic directs the beam, and the emission filter passes the fluorescence signal to the detector.

Có thể tùy chỉnh bộ lọc PCR cho thiết bị OEM không?

Yes. Wavelength, bandwidth, OD blocking, size, thickness, and channel combinations can be adjusted for OEM optical layouts.

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