How to choose OD for qPCR detection
Use stronger blocking when neighboring dye channels are close or detector gain is high. For routine qPCR, confirm both in-band transmission and out-of-band rejection before changing a standard filter set.
PCR Optical Filters for qPCR Instruments
Compare excitation filters, emission filters, and dichroic mirrors for common qPCR fluorescence channels. Use the tables to match dye channel, wavelength, FWHM, blocking level, and mechanical size before requesting a quote.
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How to Choose
Start with the dye channel and instrument optical path. Match excitation and emission wavelength first, then confirm FWHM, OD blocking, dichroic transition, angle of incidence, and filter dimensions. For multi-channel qPCR systems, keep enough spectral separation between neighboring dyes to reduce crosstalk.
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Fluorescent PCR Filters
OPTOStokes PCR filters are designed for real-time fluorescence quantitative PCR instruments including Bio-Rad CFX, Applied Biosystems QuantStudio, and Roche LightCycler. These filters provide high transmittance at target wavelengths with deep out-of-band blocking for accurate fluorescence detection in multi-channel PCR systems. Use the fluorophore-to-filter mapping table below to quickly identify the right filters for FAM, HEX, CY5, ROX, and more.
Fluorophore Channel Mapping
| Fluorophore / Dye Family | Typical Excitation Filter | Typical Emission Filter | Typical Dichroic Mirror |
|---|---|---|---|
| ATTO 425 | BP430/24 | BP480/20 or BP480/30 | DM450 |
| FAM / FITC / SYBR Green | BP470/30, BP480/20, or BP485/20 | BP520/20 or BP525/20 | DM505 |
| HEX / VIC / JOE | BP520/20, BP525/20, or BP530/20 | BP565/22 or BP570/20 | DM550 |
| CY3 / TAMRA | BP543/20 | BP580/20 or BP585/20 | DM565 |
| ROX | BP565/22, BP570/20, BP580/20, or BP585/20 | BP615/40, BP612/20, or BP620/20 | DM590 or DM600 |
| ATTO 633 | BP620/20 | BP655/20 | DM635 |
| CY5 | BP624/40 or BP630/20 | BP694/40, BP675/20, or BP680/20 | DM647 |
| CY5.5 | BP675/20 or BP665/22* | BP725/30 or BP725/40 | DM700 |
| CY7 | BP715/70, BP725/30, or BP725/40 | BP800/30 or BP800/60 | DM770 |
* CY5.5 channel: BP665/22 vs BP675/20 requires internal verification before publication. See detailed filter sets table below for complete combinations.
PCR Bandpass Filters
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| Model | CWL (nm) | FWHM (nm) | Transmittance | OD (In-band) | OD (Out-of-band) | Dimension |
|---|---|---|---|---|---|---|
| BP430/24 | 430±2 | 24±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP470/30 | 470±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP480/20 | 480±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP480/30 | 480±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP485/20 | 485±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP520/20 | 520±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP523/20 | 523±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP525/20 | 525±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP530/20 | 530±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP543/20 | 543±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP565/20 | 565±2 | 22±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP570/20 | 570±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP580/20 | 580±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP585/20 | 585±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP612/20 | 612±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP615/40 | 615±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP620/20 | 620±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP624/40 | 624±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP630/20 | 630±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP655/20 | 655±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP665/20 | 665±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP675/20 | 675±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP680/20 | 680±2 | 20±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP680/15 | 680±2 | 15±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP694/40 | 694±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP715/70 | 715±2 | 70±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP725/30 | 725±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP725/40 | 725±2 | 40±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP800/30 | 800±2 | 30±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
| BP800/60 | 800±2 | 60±2 | Tave≥90% | ODave>6@350-800 | OD>3@800-1050 | 3mm×3mm, 4mm×4mm, 6mm×6mm, 8mm×8mm, 12mm×12mm, Ø6mm, Ø8mm, Ø12mm, Ø12.7mm, Ø25mm, Ø25.4mm |
PCR Dichroic Mirrors
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| Model | AOI | Transmittance | Transmission Range (nm) | Reflectivity | Reflection Range (nm) |
|---|---|---|---|---|---|
| DM505 | 45-deg | Tave≥95% | 515-700 | R≥98% | 450-490 |
| DM550 | 45-deg | Tave≥95% | 560-700 | R≥98% | 450-540 |
| DM565 | 45-deg | Tave≥95% | 575-700 | R≥98% | 450-555 |
| DM590 | 45-deg | Tave≥95% | 603-700 | R≥98% | 450-580 |
| DM600 | 45-deg | Tave≥95% | 622-700 | R≥98% | 450-590 |
| DM635 | 45-deg | Tave≥95% | 650-700 | R≥98% | 450-630 |
| DM647 | 45-deg | Tave≥95% | 665-700 | R≥98% | 450-640 |
| DM700 | 45-deg | Tave≥95% | 715-800 | R≥98% | 450-685 |
| DM770 | 45-deg | Tave≥95% | 780-900 | R≥98% | 600-755 |
| DM500(SP) | 45-deg | Tave≥95% | 440-485 | R≥98% | 515-700 |
| DM550(SP) | 45-deg | Tave≥95% | 480-530 | R≥98% | 565-700 |
| DM565(SP) | 45-deg | Tave≥95% | 480-550 | R≥98% | 580-700 |
| DM600(SP) | 45-deg | Tave≥95% | 480-580 | R≥98% | 620-700 |
| DM650(SP) | 45-deg | Tave≥95% | 480-635 | R≥98% | 670-800 |
| DM690(SP) | 45-deg | Tave≥95% | 480-675 | R≥98% | 710-800 |
| DM450 | 45-deg | Tave≥95% | 465-680 | R≥98% | 380-445 |
Typical PCR Filter Sets
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| Fluorochrome | Excitation Filter (nm) | Emission Filter (nm) | Dichroic Mirror (nm) |
|---|---|---|---|
| Atto425 | BP430/24 | BP480/30 | DM450 |
| Atto425 | BP430/24 | BP480/20 | DM450 |
| FAM | BP470/30 | BP520/20 | DM505 |
| FAM | BP470/30 | BP525/20 | DM505 |
| FAM | BP480/20 | BP520/20 | DM505 |
| FAM | BP485/20 | BP525/20 | DM505 |
| HEX,VIC,JOE | BP520/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP525/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP530/20 | BP565/22 | DM550 |
| HEX,VIC,JOE | BP530/20 | BP570/20 | DM550 |
| CY3,TAMRA | BP543/20 | BP580/20 | DM565 |
| CY3,TAMRA | BP543/20 | BP585/20 | DM565 |
| ROX | BP565/22 | BP615/40 | DM590 |
| ROX | BP570/20 | BP612/20 | DM590 |
| ROX | BP580/20 | BP620/20 | DM600 |
| ROX | BP585/20 | BP620/20 | DM600 |
| Atto 633 | BP620/20 | BP655/20 | DM635 |
| CY5 | BP624/40 | BP694/40 | DM647 |
| CY5 | BP624/40 | BP675/20 | DM647 |
| CY5 | BP630/20 | BP675/20 | DM647 |
| CY5 | BP630/20 | BP680/20 | DM647 |
| CY5.5 | BP675/20 | BP725/30 | DM700 |
| CY5.5 | BP665/22 | BP725/40 | DM700 |
| CY7 | BP715/70 | BP800/60 | DM770 |
| CY7 | BP725/30 | BP800/30 | DM770 |
| CY7 | BP725/40 | BP800/30 | DM770 |
Related Engineering Notes
Matching filters to instrument geometry
PCR instruments may use compact optical paths, fixed holders, and non-standard angles. Check clear aperture, thickness, and AOI before assuming a wavelength match is enough.
When custom PCR filters make sense
Custom CWL or FWHM is useful when an assay uses uncommon dyes, multiplex channels, or a legacy instrument with limited space.
FAQ
Which PCR dyes are commonly matched on this page?
The page supports common qPCR channels such as FAM, SYBR Green, HEX, VIC, JOE, ROX, CY5, CY5.5, and CY7.
Do I need excitation, emission, and dichroic filters together?
Most fluorescence instruments use a matched optical set. The excitation filter selects the light source band, the dichroic directs the beam, and the emission filter passes the fluorescence signal to the detector.
Can these PCR filters be customized for OEM instruments?
Yes. Wavelength, bandwidth, OD blocking, size, thickness, and channel combinations can be adjusted for OEM optical layouts.
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