Balancing brightness and bleed-through
A wider emission band can improve signal, but it may also collect unwanted fluorescence from nearby channels. Start from the fluorophore spectrum and detector sensitivity.
Fluorescence Microscope Excitation and Emission Filters
Review matched fluorescence filter sets for common microscopy fluorophores. The tables help compare excitation bands, emission bands, dichroic mirrors, and practical replacement options for imaging systems.
Selected Models
- Contact Us for recommended models.
Additional Fluorescence Filter Models
Additional normalized fluorescence microscopy options. Confirm the fluorophore path, blocking curve, substrate, and mechanical format before production.
| Model | Application | CWL (nm) | FWHM (nm) | Transmittance | Blocking / Optical Density | Transmission Range (nm) |
|---|---|---|---|---|---|---|
| STOS-LP515 | Fluorescence microscopy / FITC | Not specified | Not specified | T≥92% | ODavg > 6 at 200-505 nm | 525-800 |
| STOS-450NF | Fluorescence microscopy | 450 ± 8 | 50 ± 5 | T≥90% | OD ≥ 5 at 445-455 nm | 405-420&480-700 |
| STOS-460NF | Fluorescence microscopy | 460 ± 10 | 65 ± 5 | T≥90% | OD ≥ 3 at 440-480 nm | 405-425&500-700 |
| STOS-470NF | Fluorescence microscopy | 470 ± 8 | 45 ± 5 | Tave≥95% | T < 5% at 465-475 nm | 350-425&505-700 |
| STOS-485NF | Fluorescence microscopy | 485 ± 20 | 190 ± 10 | T≥90% | OD ≥ 4 at 420-550 nm | 350-380&600-1100 |
| STOS-528NF | Fluorescence microscopy | 528 ± 5 | 24 ± 5 | T≥90% | OD ≥ 2 at 527-529 nm | 400-505&560-700 |
| STOS-530NF | Fluorescence microscopy | 530 ± 10 | 90 ± 10 | Tave≥95% | OD ≥ 5 at 500-560 nm | 400-470&590-800 |
| STOS-560NF | Fluorescence microscopy | 560 ± 10 | 105 ± 10 | Tave≥95% | OD ≥ 2 at 535-585 nm | 350-480&650-750 |
| STOS-575NF | Fluorescence microscopy | 575 ± 5 | 35 ± 5 | Tave≥95% | OD ≥ 2 at 570-580 nm | 430-550&600-800 |
| STOS-590NF | Fluorescence microscopy | 590 ± 5 | 35 ± 5 | T≥90% | OD ≥ 2 at 585-595 nm | 430-560&620-800 |
| STOS-600NF | Fluorescence microscopy | 600 ± 10 | 80 ± 5 | Tave≥90% | OD ≥ 2 at 630-670 nm | 380-595&725-1100 |
| STOS-625NF | Fluorescence microscopy | 625 ± 10 | 70 ± 5 | Tave≥95% | OD ≥ 6 at 610-640 nm | 500-580&675-700 |
| STOS-730NF | Fluorescence microscopy | 730 ± 15 | 150 ± 10 | Tave≥90% | OD ≥ 6 at 675-790 nm | 420-645&825-1100 |
| STOS-765NF | Fluorescence microscopy | 765 ± 15 | 170 ± 10 | T≥90% | OD ≥ 5 at 780-820 nm | 450-665&880-1100 |
| STOS-770NF | Fluorescence microscopy | 770 ± 15 | 145 ± 10 | T≥85% | OD ≥ 5 at 710-810 nm | 450-680&870-1100 |
| STOS-775NF | Fluorescence microscopy | 775 ± 15 | 130 ± 10 | Tave≥90% | OD ≥ 3 at 730-810 nm | 415-690&850-900 |
| STOS-810NF | Fluorescence microscopy | 810 ± 20 | 200 ± 10 | T≥90% | OD ≥ 2 at 730-870 nm | 380-670&930-1100 |
| STOS-1080NF | Fluorescence microscopy | 1080 ± 10 | 60 ± 5 | Tave≥95% | OD ≥ 2 at 1075-1085 nm | 900-1040&1120-1700 |
How to Choose
Choose the fluorophore first, then check whether the excitation and emission passbands fit the dye spectrum and light source. Confirm dichroic transition, microscope holder size, filter thickness, and whether neighboring channels need tighter blocking.
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Fluorescence Microscope Filters
OPTOStokes fluorescence microscope filters support common fluorophores used in widefield and confocal microscopy, including DAPI, FITC/EGFP, TRITC/PE, and CY5. This page lists individual excitation and emission filters by channel; a complete microscope configuration can add a matched dichroic mirror selected separately for the instrument and optical path.
Fluorophore Channel Mapping
Choose the fluorophore first, then review the matched excitation and emission filters. Shared models are listed once in the product table and may serve more than one channel.
| Fluorophore | Excitation Model | Excitation CWL / FWHM | Emission Model | Emission CWL / FWHM |
|---|---|---|---|---|
| DAPI | STO-375-28 | 375 ± 3 nm / 28 ± 3 nm | STO-460-55 | 460 ± 3 nm / 55 ± 3 nm |
| CFP | STO-430-24 | 430 ± 3 nm / 24 ± 3 nm | STO-480-30 | 480 ± 3 nm / 30 ± 3 nm |
| FITC | STO-470-40 | 470 ± 3 nm / 40 ± 3 nm | STO-535-40 | 535 ± 3 nm / 40 ± 3 nm |
| GFP | STO-480-30 | 480 ± 3 nm / 30 ± 3 nm | STO-535-40 | 535 ± 3 nm / 40 ± 3 nm |
| YFP | STO-485-20 | 485 ± 3 nm / 20 ± 3 nm | STO-530-20 | 530 ± 3 nm / 20 ± 3 nm |
| CY3/TRITC | STO-530-40 | 530 ± 3 nm / 40 ± 3 nm | STO-605-55 | 605 ± 3 nm / 55 ± 3 nm |
| mCherry | STO-560-40 | 560 ± 3 nm / 40 ± 3 nm | STO-635-60 | 635 ± 3 nm / 60 ± 3 nm |
| CY5 | STO-624-40 | 624 ± 3 nm / 40 ± 3 nm | STO-694-40 | 694 ± 3 nm / 40 ± 3 nm |
| CY5.5 | STO-665-30 | 665 ± 3 nm / 30 ± 3 nm | STO-725-40 | 725 ± 3 nm / 40 ± 3 nm |
| CY7 | STO-715-70 | 715 ± 3 nm / 70 ± 3 nm | STO-800-60 | 800 ± 3 nm / 60 ± 3 nm |
Fluorescence Microscope Bandpass Models
The latest reviewed source contains ten application channels and eighteen unique bandpass models. Public values use the confirmed microscopy transmission specification of T > 91%.
| Model | Role / Source Channel | Fluorophore Channels | CWL (nm) | FWHM (nm) | Transmittance (T) | Blocking | Standard Formats | Maximum Size (mm) |
|---|---|---|---|---|---|---|---|---|
| STO-375-28 | Excitation — DAPI | DAPI | 375 ± 3 | 28 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-430-24 | Excitation — CFP | CFP | 430 ± 3 | 24 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-460-55 | Emission — DAPI | DAPI | 460 ± 3 | 55 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-470-40 | Excitation — FITC | FITC | 470 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-480-30 | Excitation — GFP; Emission — CFP | GFP, CFP | 480 ± 3 | 30 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-485-20 | Excitation — YFP | YFP | 485 ± 3 | 20 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-530-20 | Emission — YFP | YFP | 530 ± 3 | 20 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-530-40 | Excitation — CY3/TRITC | CY3/TRITC | 530 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-535-40 | Emission — FITC; Emission — GFP | FITC, GFP | 535 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-560-40 | Excitation — mCherry | mCherry | 560 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-605-55 | Emission — CY3/TRITC | CY3/TRITC | 605 ± 3 | 55 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-624-40 | Excitation — CY5 | CY5 | 624 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-635-60 | Emission — mCherry | mCherry | 635 ± 3 | 60 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-665-30 | Excitation — CY5.5 | CY5.5 | 665 ± 3 | 30 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-694-40 | Emission — CY5 | CY5 | 694 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-715-70 | Excitation — CY7 | CY7 | 715 ± 3 | 70 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-725-40 | Emission — CY5.5 | CY5.5 | 725 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-800-60 | Emission — CY7 | CY7 | 800 ± 3 | 60 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 |
Shared Mechanical Format Reference
Mechanical variants are listed once here and shared by the microscope filter models above. Review the optical performance first, then select the format that matches your instrument.
| Format Family | Standard Format | Shape | Mounting | Clear Aperture | Retainer Tolerance | Size Tolerance | Substrate | Applies To |
|---|---|---|---|---|---|---|---|---|
| 1 standard format · Round · mounted · 1.0 mm substrate | Ø25 × 3.5 mm, mounted | Round | mounted | Ø22.0 mm | +0.0/-0.1 mm | Not specified | 1.0 mm (±0.1 mm) | 18 fluorescence microscope models |
Typical configuration: Ø25 × 3.5 mm mounted filter, Ø22.0 mm clear aperture, 1.0 mm substrate, and 57 × 57 mm maximum available size where specified by the source.
Related Engineering Notes
DAPI, FITC, TRITC, and CY5 selection notes
Common microscope filter sets should be checked against the light source, camera response, and dichroic transition rather than dye name alone.
Why dichroic edge placement matters
A dichroic transition that is too close to the emission band can reduce useful signal or increase channel leakage in multi-color imaging.
FAQ
What is included in a fluorescence filter set?
This catalog page lists individual excitation and emission filters by fluorophore. A complete microscope configuration may add a matched dichroic mirror selected separately for the instrument and optical path.
Can I replace filters in an existing microscope cube?
Often yes, if diameter, thickness, and optical orientation match the holder. Confirm mechanical dimensions before ordering.
Which fluorophores are covered?
The page covers common channels such as DAPI, FITC, EGFP, TRITC, PE, CY5, and related microscopy dyes.
Quality & Compliance
Documentation-ready optical filter supply
For OEM programs, laboratory instruments, and industrial systems, OPTOStokes keeps quality, material compliance, and engineering support visible from the start of supplier evaluation.
Quality system
ISO 9001 managed production
Process control, inspection records, and repeatable coating workflows for production optical components.
Material compliance
RoHS / REACH support
Compliance documentation can be prepared for qualified projects and regulated supply chains.
Factory-direct
Custom coating and dimensions
Wavelength, FWHM, substrate, AOI, size, and mounting requirements can be reviewed with engineering.
Procurement ready
Quote and data review
Share target wavelength, blocking level, transmittance, and drawing requirements for a focused quote.
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