Balancing brightness and bleed-through
A wider emission band can improve signal, but it may also collect unwanted fluorescence from nearby channels. Start from the fluorophore spectrum and detector sensitivity.
Fluorescence Filter Sets for Microscopy
Review matched fluorescence filter sets for common microscopy fluorophores. The tables help compare excitation bands, emission bands, dichroic mirrors, and practical replacement options for imaging systems.
12+ Years Experience
|
ISO 9001 Certified
|
Shipped to 60+ Countries
|
99.8% Quality Rate
Selected Models
- Contact us for recommended models.
How to Choose
Choose the fluorophore first, then check whether the excitation and emission passbands fit the dye spectrum and light source. Confirm dichroic transition, microscope holder size, filter thickness, and whether neighboring channels need tighter blocking.
2
Fluorescence Microscope Filters
OPTOStokes fluorescence microscope filter sets are designed for common fluorophores used in widefield and confocal microscopy including DAPI, FITC/EGFP, TRITC/PE, and CY5. Each filter set includes a matched excitation filter, emission filter, and dichroic mirror for optimal fluorescence imaging. Browse the pre-configured filter sets below or mix individual filters for custom configurations.
View full Fluorescence Filter Sets
Microscope Bandpass Filters
–
Showing 0 of 0
| Model | CWL (nm) | Transmittance | FWHM (nm) | OD (In-band) | OD (Out-of-band) | Dimension |
|---|---|---|---|---|---|---|
| BP375/28 | 375±2 | Tave≥85% | 28±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP430/24 | 430±2 | Tave≥90% | 24±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP460/55 | 460±2 | Tave≥90% | 55±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP470/40 | 470±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP480/30 | 480±2 | Tave≥90% | 30±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP530/40 | 530±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP535/40 | 535±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP605/55 | 605±2 | Tave≥90% | 55±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP624/40 | 624±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP694/40 | 694±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 |
Typical Microscope Filter Sets
Showing 0 of 0
| Fluorophore | Excitation Filter (nm) | Emission Filter (nm) | Dichroic Mirror (nm) |
|---|---|---|---|
| DAPI | BP375/28 | BP460/55 | DM415 |
| Atto425/ECFP | BP430/24 | BP480/30 | DM450 |
| FITC/EGFP | BP470/40 | BP535/40 | DM505 |
| FITC/EGFP | BP480/30 | BP535/40 | DM505 |
| TRITC/PE | BP530/40 | BP605/55 | DM565 |
| CY5 | BP624/40 | BP694/40 | DM660 |
Related Engineering Notes
DAPI, FITC, TRITC, and CY5 selection notes
Common microscope filter sets should be checked against the light source, camera response, and dichroic transition rather than dye name alone.
Why dichroic edge placement matters
A dichroic transition that is too close to the emission band can reduce useful signal or increase channel leakage in multi-color imaging.
FAQ
What is included in a fluorescence filter set?
A typical set includes an excitation filter, an emission filter, and a dichroic mirror matched to a fluorophore or channel group.
Can I replace filters in an existing microscope cube?
Often yes, if diameter, thickness, and optical orientation match the holder. Confirm mechanical dimensions before ordering.
Which fluorophores are covered?
The page covers common channels such as DAPI, FITC, EGFP, TRITC, PE, CY5, and related microscopy dyes.
Quality & Compliance
Documentation-ready optical filter supply
For OEM programs, laboratory instruments, and industrial systems, OPTOStokes keeps quality, material compliance, and engineering support visible from the start of supplier evaluation.
Quality system
ISO 9001 managed production
Process control, inspection records, and repeatable coating workflows for production optical components.
Material compliance
RoHS / REACH support
Compliance documentation can be prepared for qualified projects and regulated supply chains.
Factory-direct
Custom coating and dimensions
Wavelength, FWHM, substrate, AOI, size, and mounting requirements can be reviewed with engineering.
Procurement ready
Quote and data review
Share target wavelength, blocking level, transmittance, and drawing requirements for a focused quote.
Ready to Find Your Filter?
Our engineers will help you select the perfect optical solution.