Abwägung von Helligkeit und Übersprechen
A wider emission band can improve signal, but it may also collect unwanted fluorescence from nearby channels. Start from the fluorophore spectrum and detector sensitivity.
Fluoreszenzfiltersets für Mikroskopie
Prüfen Sie abgestimmte Fluoreszenzfiltersets für gängige Mikroskopie-Fluorophore. Die Tabellen helfen beim Vergleich von Anregungsbändern, Emissionsbändern, dichroitischen Spiegeln und praktischen Ersatz-Optionen für Bildgebungssysteme.
Ausgewählte Modelle
- Für empfohlene Modelle kontaktieren Sie uns.
Auswahlhilfe
Wählen Sie zuerst den Fluorophor und prüfen Sie dann, ob die Anregungs- und Emissions-Passbänder zum Farbstoffspektrum und zur Lichtquelle passen. Bestätigen Sie den dichroitischen Übergang, die Mikroskop-Fassungsgröße, Filterdicke und ob benachbarte Kanäle eine engere Sperrung erfordern.
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Fluoreszenzmikroskop-Filter
OPTOStokes Fluoreszenzmikroskop-Filtersets sind für gängige Fluorophore in Weitfeld- und Konfokalmikroskopie konzipiert, darunter DAPI, FITC/EGFP, TRITC/PE und CY5. Jedes Filterset enthält ein abgestimmtes Anregungsfilter, Emissionsfilter und einen dichroitischen Spiegel für optimale Fluoreszenzbildgebung. Durchsuchen Sie die vorkonfigurierten Filtersets oder kombinieren Sie einzelne Filter für kundenspezifische Konfigurationen.
View full Fluorescence Filter Sets
Fluorophor-Kanalzuordnung
Choose the fluorophore first, then review the matched excitation and emission filters. Shared models are listed once in the product table and may serve more than one channel.
| Farbstoffkanal | Anregungsfilter | Anregung CWL / FWHM | Emissionsfilter | Emission CWL / FWHM |
|---|---|---|---|---|
| DAPI | STO-375-28 | 375 ± 3 nm / 28 ± 3 nm | STO-460-55 | 460 ± 3 nm / 55 ± 3 nm |
| CFP | STO-430-24 | 430 ± 3 nm / 24 ± 3 nm | STO-480-30 | 480 ± 3 nm / 30 ± 3 nm |
| FITC | STO-470-40 | 470 ± 3 nm / 40 ± 3 nm | STO-535-40 | 535 ± 3 nm / 40 ± 3 nm |
| GFP | STO-480-30 | 480 ± 3 nm / 30 ± 3 nm | STO-535-40 | 535 ± 3 nm / 40 ± 3 nm |
| YFP | STO-485-20 | 485 ± 3 nm / 20 ± 3 nm | STO-530-20 | 530 ± 3 nm / 20 ± 3 nm |
| CY3/TRITC | STO-530-40 | 530 ± 3 nm / 40 ± 3 nm | STO-605-55 | 605 ± 3 nm / 55 ± 3 nm |
| mCherry | STO-560-40 | 560 ± 3 nm / 40 ± 3 nm | STO-635-60 | 635 ± 3 nm / 60 ± 3 nm |
| CY5 | STO-624-40 | 624 ± 3 nm / 40 ± 3 nm | STO-694-40 | 694 ± 3 nm / 40 ± 3 nm |
| CY5.5 | STO-665-30 | 665 ± 3 nm / 30 ± 3 nm | STO-725-40 | 725 ± 3 nm / 40 ± 3 nm |
| CY7 | STO-715-70 | 715 ± 3 nm / 70 ± 3 nm | STO-800-60 | 800 ± 3 nm / 60 ± 3 nm |
Bandpassmodelle für Fluoreszenzmikroskope
The latest reviewed source contains ten application channels and eighteen unique bandpass models. Public values use the confirmed microscopy transmission specification of T > 91%.
| Modell | Anwendungsbereich | Fluorophorkanäle | CWL (nm) | FWHM (nm) | Transmission | Sperrung | Standardformate | Maximale Größe |
|---|---|---|---|---|---|---|---|---|
| STO-375-28 | Anregung — DAPI | DAPI | 375 ± 3 | 28 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-430-24 | Anregung — CFP | CFP | 430 ± 3 | 24 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-460-55 | Emission — DAPI | DAPI | 460 ± 3 | 55 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-470-40 | Anregung — FITC | FITC | 470 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-480-30 | Anregung — GFP; Emission — CFP | GFP, CFP | 480 ± 3 | 30 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-485-20 | Anregung — YFP | YFP | 485 ± 3 | 20 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-530-20 | Emission — YFP | YFP | 530 ± 3 | 20 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-530-40 | Anregung — CY3/TRITC | CY3/TRITC | 530 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-535-40 | Emission — FITC; Emission — GFP | FITC, GFP | 535 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-560-40 | Anregung — mCherry | mCherry | 560 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-605-55 | Emission — CY3/TRITC | CY3/TRITC | 605 ± 3 | 55 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-624-40 | Anregung — CY5 | CY5 | 624 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-635-60 | Emission — mCherry | mCherry | 635 ± 3 | 60 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-665-30 | Anregung — CY5.5 | CY5.5 | 665 ± 3 | 30 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-694-40 | Emission — CY5 | CY5 | 694 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-715-70 | Anregung — CY7 | CY7 | 715 ± 3 | 70 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-725-40 | Emission — CY5.5 | CY5.5 | 725 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 | |
| STO-800-60 | Emission — CY7 | CY7 | 800 ± 3 | 60 ± 3 | T>91% | ODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) | 57 × 57 |
Gemeinsame mechanische Referenz
Mechanische Varianten werden hier einmal aufgeführt und von den oben genannten Fluoreszenzmikroskop-Filtern gemeinsam genutzt. Prüfen Sie zuerst die optische Leistung und wählen Sie anschließend das für Ihr Instrument passende Format.
| Formatfamilie | Standardformat | Form | Montage | Freie Apertur | Haltering-Toleranz | Maßtoleranz | Substrat | Gilt für |
|---|---|---|---|---|---|---|---|---|
| 1 standard format · Round · mounted · 1.0 mm substrate | Ø25 × 3.5 mm, mounted | Round | mounted | Ø22.0 mm | +0.0/-0.1 mm | Not specified | 1.0 mm (±0.1 mm) | 18 fluorescence microscope models |
Typical configuration: Ø25 × 3.5 mm mounted filter, Ø22.0 mm clear aperture, 1.0 mm substrate, and 57 × 57 mm maximum available size where specified by the source.
Verwandte technische Hinweise
Auswahltipps für DAPI, FITC, TRITC und CY5
Common microscope filter sets should be checked against the light source, camera response, and dichroic transition rather than dye name alone.
Warum die Position der dichroitischen Kante wichtig ist
A dichroic transition that is too close to the emission band can reduce useful signal or increase channel leakage in multi-color imaging.
FAQ
Was ist in einem Fluoreszenzfilterset enthalten?
This catalog page lists individual excitation and emission filters by fluorophore. A complete microscope configuration may add a matched dichroic mirror selected separately for the instrument and optical path.
Kann ich Filter in einem bestehenden Mikroskopwürfel austauschen?
Often yes, if diameter, thickness, and optical orientation match the holder. Confirm mechanical dimensions before ordering.
Welche Fluorophore werden abgedeckt?
The page covers common channels such as DAPI, FITC, EGFP, TRITC, PE, CY5, and related microscopy dyes.
Quality & Compliance
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For OEM programs, laboratory instruments, and industrial systems, OPTOStokes keeps quality, material compliance, and engineering support visible from the start of supplier evaluation.
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Process control, inspection records, and repeatable coating workflows for production optical components.
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Compliance documentation can be prepared for qualified projects and regulated supply chains.
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Custom coating and dimensions
Wavelength, FWHM, substrate, AOI, size, and mounting requirements can be reviewed with engineering.
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Share target wavelength, blocking level, transmittance, and drawing requirements for a focused quote.
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