輝度とブリードスルーのバランス調整
A wider emission band can improve signal, but it may also collect unwanted fluorescence from nearby channels. Start from the fluorophore spectrum and detector sensitivity.
顕微鏡用蛍光フィルターセット
一般的な顕微鏡蛍光色素向けマッチング蛍光フィルターセットを確認。励起帯域・蛍光帯域・ダイクロイックミラー・実用的な交換オプションを比較できます。
12年以上の実績
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ISO 9001 認証取得
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60カ国以上に出荷
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品質率 99.8%
選定型番
- Contact Us for recommended models.
選び方
まず蛍光色素を選び、励起・蛍光透過帯域が色素スペクトルと光源に合うか確認してください。ダイクロイック遷移・顕微鏡ホルダーサイズ・フィルター厚さ・隣接チャンネルの遮光要件も確認します。
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蛍光顕微鏡フィルター
OPTOStokes 蛍光顕微鏡フィルターセットは、広視野・共焦点顕微鏡で一般的な DAPI・FITC/EGFP・TRITC/PE・CY5 などの蛍光色素向けに設計されています。各セットには最適な蛍光イメージングのためのマッチングされた励起フィルター・蛍光フィルター・ダイクロイックミラーが含まれます。下記のプリセットフィルターセットを参照するか、カスタム構成で個別フィルターを組み合わせてください。
View full Fluorescence Filter Sets
Microscope Bandpass Filters
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| Model | 中心波長 (nm) | 透過率 | 半値全幅 (nm) | OD (In-band) | OD (Out-of-band) | Dimension |
|---|---|---|---|---|---|---|
| BP375/28 | 375±2 | Tave≥85% | 28±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP430/24 | 430±2 | Tave≥90% | 24±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP460/55 | 460±2 | Tave≥90% | 55±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP470/40 | 470±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP480/30 | 480±2 | Tave≥90% | 30±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP530/40 | 530±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP535/40 | 535±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP605/55 | 605±2 | Tave≥90% | 55±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP624/40 | 624±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP694/40 | 694±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 |
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| Fluorophore | Excitation Filter (nm) | Emission Filter (nm) | Dichroic Mirror (nm) |
|---|---|---|---|
| DAPI | BP375/28 | BP460/55 | DM415 |
| Atto425/ECFP | BP430/24 | BP480/30 | DM450 |
| FITC/EGFP | BP470/40 | BP535/40 | DM505 |
| FITC/EGFP | BP480/30 | BP535/40 | DM505 |
| TRITC/PE | BP530/40 | BP605/55 | DM565 |
| CY5 | BP624/40 | BP694/40 | DM660 |
関連技術資料
DAPI・FITC・TRITC・CY5の選定ポイント
Common microscope filter sets should be checked against the light source, camera response, and dichroic transition rather than dye name alone.
ダイクロイックエッジ位置が重要な理由
A dichroic transition that is too close to the emission band can reduce useful signal or increase channel leakage in multi-color imaging.
よくある質問
蛍光フィルターセットに含まれるものは？
A typical set includes an excitation filter, an emission filter, and a dichroic mirror matched to a fluorophore or channel group.
既存の顕微鏡キューブ内のフィルターを交換できますか？
Often yes, if diameter, thickness, and optical orientation match the holder. Confirm mechanical dimensions before ordering.
対応蛍光色素は？
The page covers common channels such as DAPI, FITC, EGFP, TRITC, PE, CY5, and related microscopy dyes.
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