輝度とブリードスルーのバランス調整
A wider emission band can improve signal, but it may also collect unwanted fluorescence from nearby channels. Start from the fluorophore spectrum and detector sensitivity.
顕微鏡用蛍光フィルターセット
一般的な顕微鏡蛍光色素向けマッチング蛍光フィルターセットを確認。励起帯域・蛍光帯域・ダイクロイックミラー・実用的な交換オプションを比較できます。
選定型番
- 推奨モデルについてはお問い合わせください。
追加の蛍光フィルターモデル
蛍光顕微鏡用の追加正規化候補です。生産前に蛍光色素光路、遮光曲線、基板、機械仕様を確認してください。
追加の蛍光フィルターモデル
| モデル | 用途 | 中心波長 (nm) | 半値全幅 (nm) | 透過率 | 遮光 | 透過範囲 (nm) |
|---|---|---|---|---|---|---|
| STOS-LP515 | Fluorescence microscopy / FITC | 記載なし | 記載なし | T≥92% | ODavg > 6 at 200-505 nm | 525-800 |
| STOS-450NF | Fluorescence microscopy | 450 ± 8 | 50 ± 5 | T≥90% | OD ≥ 5 at 445-455 nm | 405-420&480-700 |
| STOS-460NF | Fluorescence microscopy | 460 ± 10 | 65 ± 5 | T≥90% | OD ≥ 3 at 440-480 nm | 405-425&500-700 |
| STOS-470NF | Fluorescence microscopy | 470 ± 8 | 45 ± 5 | Tave≥95% | T < 5% at 465-475 nm | 350-425&505-700 |
| STOS-485NF | Fluorescence microscopy | 485 ± 20 | 190 ± 10 | T≥90% | OD ≥ 4 at 420-550 nm | 350-380&600-1100 |
| STOS-528NF | Fluorescence microscopy | 528 ± 5 | 24 ± 5 | T≥90% | OD ≥ 2 at 527-529 nm | 400-505&560-700 |
| STOS-530NF | Fluorescence microscopy | 530 ± 10 | 90 ± 10 | Tave≥95% | OD ≥ 5 at 500-560 nm | 400-470&590-800 |
| STOS-560NF | Fluorescence microscopy | 560 ± 10 | 105 ± 10 | Tave≥95% | OD ≥ 2 at 535-585 nm | 350-480&650-750 |
| STOS-575NF | Fluorescence microscopy | 575 ± 5 | 35 ± 5 | Tave≥95% | OD ≥ 2 at 570-580 nm | 430-550&600-800 |
| STOS-590NF | Fluorescence microscopy | 590 ± 5 | 35 ± 5 | T≥90% | OD ≥ 2 at 585-595 nm | 430-560&620-800 |
| STOS-600NF | Fluorescence microscopy | 600 ± 10 | 80 ± 5 | Tave≥90% | OD ≥ 2 at 630-670 nm | 380-595&725-1100 |
| STOS-625NF | Fluorescence microscopy | 625 ± 10 | 70 ± 5 | Tave≥95% | OD ≥ 6 at 610-640 nm | 500-580&675-700 |
| STOS-730NF | Fluorescence microscopy | 730 ± 15 | 150 ± 10 | Tave≥90% | OD ≥ 6 at 675-790 nm | 420-645&825-1100 |
| STOS-765NF | Fluorescence microscopy | 765 ± 15 | 170 ± 10 | T≥90% | OD ≥ 5 at 780-820 nm | 450-665&880-1100 |
| STOS-770NF | Fluorescence microscopy | 770 ± 15 | 145 ± 10 | T≥85% | OD ≥ 5 at 710-810 nm | 450-680&870-1100 |
| STOS-775NF | Fluorescence microscopy | 775 ± 15 | 130 ± 10 | Tave≥90% | OD ≥ 3 at 730-810 nm | 415-690&850-900 |
| STOS-810NF | Fluorescence microscopy | 810 ± 20 | 200 ± 10 | T≥90% | OD ≥ 2 at 730-870 nm | 380-670&930-1100 |
| STOS-1080NF | Fluorescence microscopy | 1080 ± 10 | 60 ± 5 | Tave≥95% | OD ≥ 2 at 1075-1085 nm | 900-1040&1120-1700 |
選び方
まず蛍光色素を選び、励起・蛍光透過帯域が色素スペクトルと光源に合うか確認してください。ダイクロイック遷移・顕微鏡ホルダーサイズ・フィルター厚さ・隣接チャンネルの遮光要件も確認します。
2
蛍光顕微鏡フィルター
OPTOStokes 蛍光顕微鏡フィルターセットは、広視野・共焦点顕微鏡で一般的な DAPI・FITC/EGFP・TRITC/PE・CY5 などの蛍光色素向けに設計されています。各セットには最適な蛍光イメージングのためのマッチングされた励起フィルター・蛍光フィルター・ダイクロイックミラーが含まれます。下記のプリセットフィルターセットを参照するか、カスタム構成で個別フィルターを組み合わせてください。
蛍光団チャンネル対応表
Choose the fluorophore first, then review the matched excitation and emission filters. Shared models are listed once in the product table and may serve more than one channel.
| 蛍光色素チャネル | 励起フィルター | 励起 CWL / FWHM | 蛍光フィルター | 蛍光 CWL / FWHM |
|---|---|---|---|---|
| DAPI | STO-375-28 | 375 ± 3 nm / 28 ± 3 nm | STO-460-55 | 460 ± 3 nm / 55 ± 3 nm |
| CFP | STO-430-24 | 430 ± 3 nm / 24 ± 3 nm | STO-480-30 | 480 ± 3 nm / 30 ± 3 nm |
| FITC | STO-470-40 | 470 ± 3 nm / 40 ± 3 nm | STO-535-40 | 535 ± 3 nm / 40 ± 3 nm |
| GFP | STO-480-30 | 480 ± 3 nm / 30 ± 3 nm | STO-535-40 | 535 ± 3 nm / 40 ± 3 nm |
| YFP | STO-485-20 | 485 ± 3 nm / 20 ± 3 nm | STO-530-20 | 530 ± 3 nm / 20 ± 3 nm |
| CY3/TRITC | STO-530-40 | 530 ± 3 nm / 40 ± 3 nm | STO-605-55 | 605 ± 3 nm / 55 ± 3 nm |
| mCherry | STO-560-40 | 560 ± 3 nm / 40 ± 3 nm | STO-635-60 | 635 ± 3 nm / 60 ± 3 nm |
| CY5 | STO-624-40 | 624 ± 3 nm / 40 ± 3 nm | STO-694-40 | 694 ± 3 nm / 40 ± 3 nm |
| CY5.5 | STO-665-30 | 665 ± 3 nm / 30 ± 3 nm | STO-725-40 | 725 ± 3 nm / 40 ± 3 nm |
| CY7 | STO-715-70 | 715 ± 3 nm / 70 ± 3 nm | STO-800-60 | 800 ± 3 nm / 60 ± 3 nm |
蛍光顕微鏡用バンドパスモデル
The latest reviewed source contains ten application channels and eighteen unique bandpass models. Public values use the confirmed microscopy transmission specification of T > 91%.
| モデル | 用途 | 蛍光色素チャネル | CWL (nm) | FWHM (nm) | 透過率 | 遮光 | 標準フォーマット | 最大サイズ |
|---|---|---|---|---|---|---|---|---|
| STO-375-28 | 励起 — DAPI | DAPI | 375 ± 3 | 28 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-430-24 | 励起 — CFP | CFP | 430 ± 3 | 24 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-460-55 | 蛍光 — DAPI | DAPI | 460 ± 3 | 55 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-470-40 | 励起 — FITC | FITC | 470 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-480-30 | 励起 — GFP; 蛍光 — CFP | GFP, CFP | 480 ± 3 | 30 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-485-20 | 励起 — YFP | YFP | 485 ± 3 | 20 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-530-20 | 蛍光 — YFP | YFP | 530 ± 3 | 20 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-530-40 | 励起 — CY3/TRITC | CY3/TRITC | 530 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-535-40 | 蛍光 — FITC; 蛍光 — GFP | FITC, GFP | 535 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-560-40 | 励起 — mCherry | mCherry | 560 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-605-55 | 蛍光 — CY3/TRITC | CY3/TRITC | 605 ± 3 | 55 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-624-40 | 励起 — CY5 | CY5 | 624 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-635-60 | 蛍光 — mCherry | mCherry | 635 ± 3 | 60 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-665-30 | 励起 — CY5.5 | CY5.5 | 665 ± 3 | 30 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-694-40 | 蛍光 — CY5 | CY5 | 694 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-715-70 | 励起 — CY7 | CY7 | 715 ± 3 | 70 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-725-40 | 蛍光 — CY5.5 | CY5.5 | 725 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-800-60 | 蛍光 — CY7 | CY7 | 800 ± 3 | 60 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nm適用条件を見るODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 |
共通機械仕様リファレンス
機械仕様のバリエーションはここにまとめて掲載し、上記の顕微鏡フィルターモデルで共有しています。まず光学性能を確認し、装置に合うフォーマットを選択してください。
| フォーマットファミリー | 標準フォーマット | 形状 | 取付方式 | 有効径 | 保持リング公差 | 寸法公差 | 基板 | 対応モデル |
|---|---|---|---|---|---|---|---|---|
| 標準仕様：1 · 円形 · マウント付き · 1.0 mm 基板 | Ø25 × 3.5 mm, マウント付き | 円形 | マウント付き | Ø22.0 mm | +0.0/-0.1 mm | 記載なし | 1.0 mm (±0.1 mm) | 蛍光顕微鏡モデル：18 |
Typical configuration: Ø25 × 3.5 mm mounted filter, Ø22.0 mm clear aperture, 1.0 mm substrate, and 57 × 57 mm maximum available size where specified by the source.
関連技術資料
DAPI・FITC・TRITC・CY5の選定ポイント
Common microscope filter sets should be checked against the light source, camera response, and dichroic transition rather than dye name alone.
ダイクロイックエッジ位置が重要な理由
A dichroic transition that is too close to the emission band can reduce useful signal or increase channel leakage in multi-color imaging.
よくある質問
蛍光フィルターセットに含まれるものは？
一般的なセットには、蛍光色素またはチャンネルグループにマッチングされた励起フィルター・蛍光フィルター・ダイクロイックミラーが含まれます。
既存の顕微鏡キューブ内のフィルターを交換できますか？
径・厚さ・光学方向がホルダーに合致すれば可能です。発注前に機械的寸法を確認してください。
対応蛍光色素は？
DAPI・FITC・EGFP・TRITC・PE・CY5 および関連顕微鏡蛍光色素の一般的なチャンネルをカバーしています。
Quality & Compliance
Documentation-ready optical filter supply
For OEM programs, laboratory instruments, and industrial systems, OPTOStokes keeps quality, material compliance, and engineering support visible from the start of supplier evaluation.
Quality system
ISO 9001 managed production
Process control, inspection records, and repeatable coating workflows for production optical components.
Material compliance
RoHS / REACH support
Compliance documentation can be prepared for qualified projects and regulated supply chains.
Factory-direct
Custom coating and dimensions
Wavelength, FWHM, substrate, AOI, size, and mounting requirements can be reviewed with engineering.
Procurement ready
Quote and data review
Share target wavelength, blocking level, transmittance, and drawing requirements for a focused quote.
フィルターをお探しですか？
Our engineers will help you select the perfect optical solution.