Equilibrio entre brillo y fuga de luz
A wider emission band can improve signal, but it may also collect unwanted fluorescence from nearby channels. Start from the fluorophore spectrum and detector sensitivity.
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Revise conjuntos de filtros de fluorescencia coincidentes para colorantes comunes de microscopio. Compare bandas de excitación, bandas de emisión, espejos dicroicos y opciones de reemplazo práctico.
Elija primero el fluoróforo, luego verifique si las bandas de paso de excitación y emisión se ajustan al espectro del colorante y la fuente de luz. Confirme la transición dicroica, el tamaño del portador del microscopio, el espesor del filtro y si los canales vecinos necesitan bloqueo más estricto.
Los juegos de filtros para microscopio de fluorescencia OPTOStokes están diseñados para fluoróforos comunes en microscopía de campo amplio y confocal, incluyendo DAPI, FITC/EGFP, TRITC/PE y CY5. Cada juego incluye un filtro de excitación, un filtro de emisión y un espejo dicroico coincidentes para una imagen de fluorescencia óptima. Explore los juegos preconfigurados o combine filtros individuales para configuraciones personalizadas.
View full Fluorescence Filter Sets
| Model | Longitud de onda central (nm) | Transmitancia | Ancho a mitad de altura (nm) | OD (In-band) | OD (Out-of-band) | Dimension |
|---|---|---|---|---|---|---|
| BP375/28 | 375±2 | Tave≥85% | 28±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP430/24 | 430±2 | Tave≥90% | 24±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP460/55 | 460±2 | Tave≥90% | 55±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP470/40 | 470±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP480/30 | 480±2 | Tave≥90% | 30±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP530/40 | 530±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP535/40 | 535±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP605/55 | 605±2 | Tave≥90% | 55±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP624/40 | 624±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 | |
| BP694/40 | 694±2 | Tave≥90% | 40±2 | ODave>6@350-800 | OD>3@800-1050 |
| Fluorophore | Excitation Filter (nm) | Emission Filter (nm) | Dichroic Mirror (nm) |
|---|---|---|---|
| DAPI | BP375/28 | BP460/55 | DM415 |
| Atto425/ECFP | BP430/24 | BP480/30 | DM450 |
| FITC/EGFP | BP470/40 | BP535/40 | DM505 |
| FITC/EGFP | BP480/30 | BP535/40 | DM505 |
| TRITC/PE | BP530/40 | BP605/55 | DM565 |
| CY5 | BP624/40 | BP694/40 | DM660 |
A wider emission band can improve signal, but it may also collect unwanted fluorescence from nearby channels. Start from the fluorophore spectrum and detector sensitivity.
Common microscope filter sets should be checked against the light source, camera response, and dichroic transition rather than dye name alone.
A dichroic transition that is too close to the emission band can reduce useful signal or increase channel leakage in multi-color imaging.
A typical set includes an excitation filter, an emission filter, and a dichroic mirror matched to a fluorophore or channel group.
Often yes, if diameter, thickness, and optical orientation match the holder. Confirm mechanical dimensions before ordering.
The page covers common channels such as DAPI, FITC, EGFP, TRITC, PE, CY5, and related microscopy dyes.
Quality & Compliance
For OEM programs, laboratory instruments, and industrial systems, OPTOStokes keeps quality, material compliance, and engineering support visible from the start of supplier evaluation.
Process control, inspection records, and repeatable coating workflows for production optical components.
Compliance documentation can be prepared for qualified projects and regulated supply chains.
Wavelength, FWHM, substrate, AOI, size, and mounting requirements can be reviewed with engineering.
Share target wavelength, blocking level, transmittance, and drawing requirements for a focused quote.
Our engineers will help you select the perfect optical solution.
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