Equilibrio entre brillo y fuga de luz
A wider emission band can improve signal, but it may also collect unwanted fluorescence from nearby channels. Start from the fluorophore spectrum and detector sensitivity.
Juegos de filtros de fluorescencia para microscopía
Revise conjuntos de filtros de fluorescencia coincidentes para colorantes comunes de microscopio. Compare bandas de excitación, bandas de emisión, espejos dicroicos y opciones de reemplazo práctico.
Modelos seleccionados
- Contáctenos para obtener modelos recomendados.
Modelos adicionales de filtros de fluorescencia
Opciones adicionales normalizadas para microscopía de fluorescencia. Confirme la ruta del fluoróforo, la curva de bloqueo, el sustrato y el formato mecánico antes de la producción.
Modelos adicionales de filtros de fluorescencia
| Modelo | Aplicación | Longitud de onda central (nm) | Ancho FWHM (nm) | Transmitancia | Bloqueo | Rango de transmisión (nm) |
|---|---|---|---|---|---|---|
| STOS-LP515 | Fluorescence microscopy / FITC | No especificado | No especificado | T≥92% | ODavg > 6 at 200-505 nm | 525-800 |
| STOS-450NF | Fluorescence microscopy | 450 ± 8 | 50 ± 5 | T≥90% | OD ≥ 5 at 445-455 nm | 405-420&480-700 |
| STOS-460NF | Fluorescence microscopy | 460 ± 10 | 65 ± 5 | T≥90% | OD ≥ 3 at 440-480 nm | 405-425&500-700 |
| STOS-470NF | Fluorescence microscopy | 470 ± 8 | 45 ± 5 | Tave≥95% | T < 5% at 465-475 nm | 350-425&505-700 |
| STOS-485NF | Fluorescence microscopy | 485 ± 20 | 190 ± 10 | T≥90% | OD ≥ 4 at 420-550 nm | 350-380&600-1100 |
| STOS-528NF | Fluorescence microscopy | 528 ± 5 | 24 ± 5 | T≥90% | OD ≥ 2 at 527-529 nm | 400-505&560-700 |
| STOS-530NF | Fluorescence microscopy | 530 ± 10 | 90 ± 10 | Tave≥95% | OD ≥ 5 at 500-560 nm | 400-470&590-800 |
| STOS-560NF | Fluorescence microscopy | 560 ± 10 | 105 ± 10 | Tave≥95% | OD ≥ 2 at 535-585 nm | 350-480&650-750 |
| STOS-575NF | Fluorescence microscopy | 575 ± 5 | 35 ± 5 | Tave≥95% | OD ≥ 2 at 570-580 nm | 430-550&600-800 |
| STOS-590NF | Fluorescence microscopy | 590 ± 5 | 35 ± 5 | T≥90% | OD ≥ 2 at 585-595 nm | 430-560&620-800 |
| STOS-600NF | Fluorescence microscopy | 600 ± 10 | 80 ± 5 | Tave≥90% | OD ≥ 2 at 630-670 nm | 380-595&725-1100 |
| STOS-625NF | Fluorescence microscopy | 625 ± 10 | 70 ± 5 | Tave≥95% | OD ≥ 6 at 610-640 nm | 500-580&675-700 |
| STOS-730NF | Fluorescence microscopy | 730 ± 15 | 150 ± 10 | Tave≥90% | OD ≥ 6 at 675-790 nm | 420-645&825-1100 |
| STOS-765NF | Fluorescence microscopy | 765 ± 15 | 170 ± 10 | T≥90% | OD ≥ 5 at 780-820 nm | 450-665&880-1100 |
| STOS-770NF | Fluorescence microscopy | 770 ± 15 | 145 ± 10 | T≥85% | OD ≥ 5 at 710-810 nm | 450-680&870-1100 |
| STOS-775NF | Fluorescence microscopy | 775 ± 15 | 130 ± 10 | Tave≥90% | OD ≥ 3 at 730-810 nm | 415-690&850-900 |
| STOS-810NF | Fluorescence microscopy | 810 ± 20 | 200 ± 10 | T≥90% | OD ≥ 2 at 730-870 nm | 380-670&930-1100 |
| STOS-1080NF | Fluorescence microscopy | 1080 ± 10 | 60 ± 5 | Tave≥95% | OD ≥ 2 at 1075-1085 nm | 900-1040&1120-1700 |
Cómo elegir
Elija primero el fluoróforo, luego verifique si las bandas de paso de excitación y emisión se ajustan al espectro del colorante y la fuente de luz. Confirme la transición dicroica, el tamaño del portador del microscopio, el espesor del filtro y si los canales vecinos necesitan bloqueo más estricto.
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Filtros para microscopio de fluorescencia
Los juegos de filtros para microscopio de fluorescencia OPTOStokes están diseñados para fluoróforos comunes en microscopía de campo amplio y confocal, incluyendo DAPI, FITC/EGFP, TRITC/PE y CY5. Cada juego incluye un filtro de excitación, un filtro de emisión y un espejo dicroico coincidentes para una imagen de fluorescencia óptima. Explore los juegos preconfigurados o combine filtros individuales para configuraciones personalizadas.
Mapa de canales de fluoróforos
Choose the fluorophore first, then review the matched excitation and emission filters. Shared models are listed once in the product table and may serve more than one channel.
| Canal de fluoróforo | Filtro de excitación | CWL / FWHM de excitación | Filtro de emisión | CWL / FWHM de emisión |
|---|---|---|---|---|
| DAPI | STO-375-28 | 375 ± 3 nm / 28 ± 3 nm | STO-460-55 | 460 ± 3 nm / 55 ± 3 nm |
| CFP | STO-430-24 | 430 ± 3 nm / 24 ± 3 nm | STO-480-30 | 480 ± 3 nm / 30 ± 3 nm |
| FITC | STO-470-40 | 470 ± 3 nm / 40 ± 3 nm | STO-535-40 | 535 ± 3 nm / 40 ± 3 nm |
| GFP | STO-480-30 | 480 ± 3 nm / 30 ± 3 nm | STO-535-40 | 535 ± 3 nm / 40 ± 3 nm |
| YFP | STO-485-20 | 485 ± 3 nm / 20 ± 3 nm | STO-530-20 | 530 ± 3 nm / 20 ± 3 nm |
| CY3/TRITC | STO-530-40 | 530 ± 3 nm / 40 ± 3 nm | STO-605-55 | 605 ± 3 nm / 55 ± 3 nm |
| mCherry | STO-560-40 | 560 ± 3 nm / 40 ± 3 nm | STO-635-60 | 635 ± 3 nm / 60 ± 3 nm |
| CY5 | STO-624-40 | 624 ± 3 nm / 40 ± 3 nm | STO-694-40 | 694 ± 3 nm / 40 ± 3 nm |
| CY5.5 | STO-665-30 | 665 ± 3 nm / 30 ± 3 nm | STO-725-40 | 725 ± 3 nm / 40 ± 3 nm |
| CY7 | STO-715-70 | 715 ± 3 nm / 70 ± 3 nm | STO-800-60 | 800 ± 3 nm / 60 ± 3 nm |
Modelos bandpass para microscopía de fluorescencia
The latest reviewed source contains ten application channels and eighteen unique bandpass models. Public values use the confirmed microscopy transmission specification of T > 91%.
| Modelo | Aplicación | Canales de fluoróforos | CWL (nm) | FWHM (nm) | Transmitancia | Bloqueo | Formatos estándar | Tamaño máximo |
|---|---|---|---|---|---|---|---|---|
| STO-375-28 | Excitación — DAPI | DAPI | 375 ± 3 | 28 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-430-24 | Excitación — CFP | CFP | 430 ± 3 | 24 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-460-55 | Emisión — DAPI | DAPI | 460 ± 3 | 55 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-470-40 | Excitación — FITC | FITC | 470 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-480-30 | Excitación — GFP; Emisión — CFP | GFP, CFP | 480 ± 3 | 30 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-485-20 | Excitación — YFP | YFP | 485 ± 3 | 20 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-530-20 | Emisión — YFP | YFP | 530 ± 3 | 20 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-530-40 | Excitación — CY3/TRITC | CY3/TRITC | 530 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-535-40 | Emisión — FITC; Emisión — GFP | FITC, GFP | 535 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-560-40 | Excitación — mCherry | mCherry | 560 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-605-55 | Emisión — CY3/TRITC | CY3/TRITC | 605 ± 3 | 55 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-624-40 | Excitación — CY5 | CY5 | 624 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-635-60 | Emisión — mCherry | mCherry | 635 ± 3 | 60 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-665-30 | Excitación — CY5.5 | CY5.5 | 665 ± 3 | 30 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-694-40 | Emisión — CY5 | CY5 | 694 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-715-70 | Excitación — CY7 | CY7 | 715 ± 3 | 70 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-725-40 | Emisión — CY5.5 | CY5.5 | 725 ± 3 | 40 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 | |
| STO-800-60 | Emisión — CY7 | CY7 | 800 ± 3 | 60 ± 3 | T>91% | ODavg > 6 at 350-800 nm ; OD > 3 at 800-1050 nmConsultar condicionesODavg > 6 at 350-800 nm (out-of-band; passband excluded where applicable); OD > 3 at 800-1050 nm (out-of-band; passband excluded where applicable) |
57 × 57 |
Referencia común de formatos mecánicos
Las variantes mecánicas se enumeran una sola vez aquí y se comparten entre los modelos de filtros para microscopía de fluorescencia anteriores. Revise primero el rendimiento óptico y después seleccione el formato compatible con su instrumento.
| Familia de formatos | Formato estándar | Forma | Montaje | Apertura útil | Tolerancia del retenedor | Tolerancia dimensional | Sustrato | Aplicable a |
|---|---|---|---|---|---|---|---|---|
| Formatos estándar: 1 · Redondo · montado · 1.0 mm sustrato | Ø25 × 3.5 mm, montado | Redondo | montado | Ø22.0 mm | +0.0/-0.1 mm | No especificado | 1.0 mm (±0.1 mm) | Modelos para microscopía de fluorescencia: 18 |
Typical configuration: Ø25 × 3.5 mm mounted filter, Ø22.0 mm clear aperture, 1.0 mm substrate, and 57 × 57 mm maximum available size where specified by the source.
Notas técnicas relacionadas
Notas de selección DAPI, FITC, TRITC y CY5
Common microscope filter sets should be checked against the light source, camera response, and dichroic transition rather than dye name alone.
Por qué importa la posición del borde dicroico
A dichroic transition that is too close to the emission band can reduce useful signal or increase channel leakage in multi-color imaging.
Preguntas frecuentes
¿Qué incluye un juego de filtros de fluorescencia?
Un juego típico incluye un filtro de excitación, un filtro de emisión y un espejo dicroico coincidentes con un fluoróforo o grupo de canales.
¿Puedo reemplazar filtros en un cubo de microscopio existente?
A menudo sí, si el diámetro, espesor y orientación óptica coinciden con el portador. Confirme las dimensiones mecánicas antes de ordenar.
¿Qué fluoróforos están cubiertos?
La página cubre canales comunes como DAPI, FITC, EGFP, TRITC, PE, CY5 y colorantes de microscopía relacionados.
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