OD6 blocking for weak fluorescence
In biochemical detection, residual excitation light can mask weak emission signals. OD6 blocking is often specified to reduce detector-side leakage.
Biochemical Analysis, Microplate Reader & Flow Cytometry Filters
Compare OD6 fluorescence filters for ELISA readers, microplate detection, POCT modules, PCR optical paths, and flow cytometry channels where weak signals must be separated from excitation light.
Selected Models
- STO-BP470/30N for visible fluorescence detection channels.
- STO-BP543/20N and STO-BP612/20N for narrow assay bands requiring OD6 blocking.
- STO-NBP365/10N for UV biochemical detection paths.
How to Choose
Start from the assay wavelength or fluorescence channel, then confirm CWL, FWHM, peak transmission, and OD6 blocking range. For weak fluorescence signals, blocking depth and detector-side stray-light control are often as important as peak transmittance.
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Biochemical Analysis & Flow Cytometry Filters
Microplate readers and ELISA instruments use filtered monochromatic light to measure absorbance or fluorescence in small sample volumes. For fluorescence-based biochemical analysis, POCT modules, PCR optical paths, and flow cytometry channels, OD6 blocking helps suppress excitation leakage before it reaches the detector.
Compare related Narrow Bandpass Filters
Microplate Reader and Biochemical Fluorescence Filters
| Model | Spectral Range | CWL (nm) | FWHM (nm) | Peak Transmittance | Blocking |
|---|---|---|---|---|---|
| STO-NBP365/10N | UV | 365±2 | 10±2 | T>60% | OD6 @ 200-800nm |
| STO-BP470/30N | Visible | 470±2 | 30±2 | T>70% | OD6 @ 200-800nm |
| STO-BP523/25N | Visible | 523±2 | 25±2 | T>70% | OD6 @ 200-800nm |
| STO-BP525/20N | Visible | 525±2 | 20±2 | T>70% | OD6 @ 200-800nm |
| STO-BP543/20N | Visible | 543±2 | 20±2 | T>70% | OD6 @ 200-800nm |
| STO-BP564/25N | Visible | 564±2 | 25±2 | T>70% | OD6 @ 200-800nm |
| STO-BP571/20N | Visible | 571±2 | 20±2 | T>70% | OD6 @ 200-800nm |
| STO-BP584/20N | Visible | 584±2 | 20±2 | T>70% | OD6 @ 200-800nm |
| STO-BP612/20N | Visible | 612±2 | 20±2 | T>70% | OD6 @ 200-800nm |
| STO-BP615/20N | Visible | 615±3 | 20±3 | T>70% | OD6 @ 200-800nm |
| STO-BP624/40N | Visible | 624±3 | 40±4 | T>70% | OD6 @ 200-800nm |
| STO-BP635/20N | Visible | 635±2 | 20±2 | T>70% | OD6 @ 200-800nm |
| STO-BP635/30N | Visible | 635±2 | 30±2 | T>70% | OD6 @ 200-800nm |
| STO-BP655/20N | Visible | 655±2 | 20±2 | T>70% | OD6 @ 200-800nm |
| STO-BP680/20N | Visible | 680±2 | 20±2 | T>70% | OD6 @ 200-800nm |
| STO-NBP683/15N | Visible | 683±2 | 15±2 | T>70% | OD6 @ 200-800nm |
| STO-BP692/40N | Visible | 692±3 | 40±4 | T>70% | OD6 @ 200-800nm |
| STO-BP716/20N | NIR | 716±2 | 20±2 | T>70% | OD6 @ 200-800nm |
Related Engineering Notes
Microplate reader wavelength choices
Absorbance and fluorescence assays often use channels around 405nm, 450nm, 492nm, 630nm, and related visible fluorescence bands. Confirm the assay kit and detector response before selecting a filter.
POCT and custom module support
For POCT, PCR, or compact fluorescence modules, share the optical path, light source, detector, AOI, and mechanical size so the filter stack can be reviewed as a system.
FAQ
What wavelengths are common in microplate readers?
Common channels include 405nm, 450nm, 492nm, 630nm, and assay-specific fluorescence bands depending on the detection chemistry.
Why use narrow fluorescence filters in biochemical analysis?
Narrow passbands improve wavelength discrimination while OD6 blocking helps suppress stray excitation energy before it reaches the detector.
Can OPTOStokes support POCT or PCR optical modules?
Yes. OPTOStokes can review fluorescence filters and optical module requirements for POCT, PCR, biochemical analysis, and custom detector assemblies.
Quality & Compliance
Documentation-ready optical filter supply
For OEM programs, laboratory instruments, and industrial systems, OPTOStokes keeps quality, material compliance, and engineering support visible from the start of supplier evaluation.
Quality system
ISO 9001 managed production
Process control, inspection records, and repeatable coating workflows for production optical components.
Material compliance
RoHS / REACH support
Compliance documentation can be prepared for qualified projects and regulated supply chains.
Factory-direct
Custom coating and dimensions
Wavelength, FWHM, substrate, AOI, size, and mounting requirements can be reviewed with engineering.
Procurement ready
Quote and data review
Share target wavelength, blocking level, transmittance, and drawing requirements for a focused quote.
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