Introduction to Fluorescence Microscopy

Fluorescence microscopy is a powerful tool for visualizing microscopic details in biomedical research. Optical filters, as critical components, directly impact image quality by controlling light wavelengths, especially in demanding applications like CY5 and CY5.5 imaging.

Challenges of CY5 and CY5.5 Imaging

CY5 and CY5.5 are widely used fluorescent dyes in fluorescence microscopy, flow cytometry, and in vivo imaging. Their excitation and emission wavelengths, located in the near-infrared range (approximately 650nm and 680nm for excitation, 670nm and 710nm for emission), require highly precise optical filters. Inadequate filters can weaken or fail to capture fluorescence signals, compromising image clarity.

How Fluorescence Imaging Works

Fluorescence imaging relies on the Stokes shift, where a fluorophore absorbs light at a specific wavelength and emits light at a longer wavelength. For CY5 and CY5.5, fluorescence optical filters ensure that only the desired excitation and emission wavelengths are transmitted, producing clear and accurate images.

Role of the Fluorescence Filter Cube

The fluorescence filter cube consists of three key components:

• Excitation Filter: Selects the wavelength to excite the fluorophore.
• Dichroic Mirror: Separates excitation and emission light.
• Emission Filter: Filters the emitted fluorescence signal for enhanced clarity.

These components work together to maximize signal-to-noise ratio and deliver high-quality images.

Types of Fluorescence Filters

Fluorescence filters include:

• Longpass Filters (LP): Transmit longer wavelengths, suitable for broad emission capture.
• Shortpass Filters (SP): Transmit shorter wavelengths, used in specific UV applications.
• Bandpass Filters (BP): Offer the highest signal-to-noise ratio by transmitting a narrow wavelength range, ideal for CY5 and CY5.5 imaging.

Why Choose Our Fluorescence Filters?

As a leading supplier of optical filters, we provide:

• Precision Performance: High-transmittance CY5 filter sets and bandpass filters ensure superior image clarity.
• Custom and Standard Options: Stock filters and tailored designs meet diverse application needs.
• Global Quality Standards: Our filters match international benchmarks at competitive prices, perfect for microscopy and spectroscopy.