The **OPTOStokes CY5 Fluorescence Filter Set** is engineered for superior signal resolution in the deep red to Near-Infrared (NIR) spectrum, crucial for multiplexing assays in qPCR, flow cytometry, and advanced imaging. Optimized for the CY5 fluorophore, this set delivers high transmission efficiency and exceptional blocking (Optical Density > 6), effectively isolating the target signal from background noise and unwanted excitation energy.
Technical Specifications for High-Contrast Performance
Each filter is fabricated using high-stability thin-film coatings, offering precise spectral control for demanding medical and research instrumentation.
| Component | Model / Type | Center Wavelength (CWL) | FWHM (Bandwidth) | Transmission | Blocking (OD) |
|---|---|---|---|---|---|
| Excitation Filter | BP675-20nm | 675nm ± 2nm | 20nm ± 2nm | Tave >90% (670-680nm) | ODave > 6 (350-655nm, 695-800nm) |
| Emission Filter | BP725-30nm | 725nm ± 2nm | 30nm ± 2nm | Tave >90% (715-735nm) | ODave > 6 (350-700nm, 752-830nm) |
| Dichroic Mirror | DM700 | Cut-on 700nm (nominal) | N/A | Tave > 95% (715-850nm) | Block < 2% (430-685nm) |
Primary Applications
The tight spectral tolerances and deep blocking make this filter set ideal for high-sensitivity detection in:
- Quantitative PCR (qPCR): Robust detection channel for CY5 and related dyes in multi-channel systems.
- Fluorescence Imaging: NIR filter applications demanding clear signal separation in the deep red spectrum.
- Flow Cytometry: Precision sorting and analysis of cells tagged with long-wavelength fluorophores.
- Biomedical Sensing: High-reliability optical path for PCR detection and diagnostics.
The OPTOStokes Advantage: Customization & Quality
We offer flexible manufacturing to meet specialized optical system requirements. Our custom optical filters service covers the entire spectrum of high-performance optics:
- Custom Parameters: Adjustments to CWL (400nm-850nm), FWHM (10nm-60nm), and Transmission (Tave > 90%).
- Deep Blocking Guarantee: Guaranteed absolute Optical Density (ODabs) > 5 and average ODave > 6 blocking across the critical 350nm-800nm range.
- Physical Specs: Standard thicknesses of 1.0mm/1.1mm. Available dimensions include 6x6mm, diameter 6mm, 12mm, and 25mm. Custom shapes and aluminum mounting rings are available upon request.
Reliability for Mission-Critical Systems
Relying on standard stock filters can lead to compromise in data integrity, especially in the NIR range where noise is prevalent. Our fluorescence filters provide the guaranteed high-performance, quality, and reliable lead times you need. Stop compromising on signal quality; leverage our **World-Class Precision**.
Ready to integrate high-performance high OD filter sets into your instrument? Contact our specialized engineers directly at sales@optofilters.com for technical consultation or rapid quote requests.
