In the precision-demanding field of molecular diagnostics, signal clarity is paramount. The OPTOStokes ROX Fluorescence Filter Set is engineered specifically for Real-Time PCR (qPCR) systems and fluorescence microscopy, optimized to capture the specific spectral signature of ROX dyes. By combining steep spectral edges with deep blocking capabilities, this set minimizes crosstalk and maximizes the signal-to-noise ratio, ensuring reliable data for critical R&D and diagnostic applications.
Technical Specifications
Our filters utilize hard-coated optical glass technology to ensure environmental stability and long-term performance. The set includes a matched Excitation Filter, Emission Filter, and Dichroic Mirror.
| Component | Model / Type | Center Wavelength (CWL) | FWHM (Bandwidth) | Transmission | Blocking (OD) |
|---|---|---|---|---|---|
| Excitation | BP570-20nm | 570nm ± 2nm | 20nm ± 2nm | Tave > 90% (565-575nm) | OD > 6 (350-550nm) OD > 6 (591-800nm) |
| Emission | BP612-20nm | 612nm ± 2nm | 20nm ± 2nm | Tave > 90% (607-617nm) | OD > 6 (350-591.5nm) OD > 6 (632-800nm) |
| Dichroic Mirror | DM590 | Cut-on ~590nm | N/A | T > 95% (625-850nm) | Reflects < 580nm |
Key Applications
Designed for high-sensitivity optical systems, this filter set is widely deployed in:
- Quantitative PCR (qPCR): Standard reference dye detection and multiplexing.
- Fluorescence Microscopy: High-contrast imaging of red-emitting fluorophores.
- DNA Sequencing: Precision signal separation for genetic analysis.
- Biomedical Instrumentation: PCR detection devices requiring compact, high-performance optics.
Customization & Engineering Support
At OPTOStokes, we understand that standard sizes do not fit every optical bench. We offer extensive custom optical filters services to match your mechanical constraints.
- Wavelength Options: CWL selectable from 400nm to 850nm; FWHM adjustable from 10nm to 60nm.
- Substrate & Size: Standard sizes include 6x6mm, diameter 6mm, 12mm, and 25mm. Custom shapes (square, round, irregular) and anodized aluminum rings are available.
- Optical Density: We can achieve ODabs ≥5 and ODave ≥ 6 for critical sensing applications requiring a high OD filter.
Why Choose OPTOStokes?
Eliminate Signal Noise: Our fluorescence filters feature blocking depths greater than OD6, effectively suppressing excitation light bleed-through and ensuring that only the target fluorescence signal reaches the detector.
Rapid Prototyping to Volume: Whether you need a single prototype for validation or 10,000 units for production, our stock inventory and agile manufacturing ensure on-time delivery.
Need a tailored optical solution? Contact our engineering team at sales@optofilters.com to discuss your specific requirements for bandpass filter specifications and system integration.
