BP720nm Bandpass Filter | FWHM 10nm, Thickness 1.1mm
The BP720-10nm-1.1mm bandpass filter is precision-engineered for near-infrared fluorescence applications demanding exceptional spectral selectivity in the 720nm region. This advanced bandpass filter features a narrow 10nm full width at half maximum (FWHM) centered at 720nm with transmission greater than 90% across the 716-723nm passband, providing superior wavelength discrimination for Alexa Fluor 700, Alexa Fluor 750, and other near-infrared emitting fluorophores. With comprehensive blocking from 350-1100nm at transmission less than 1%, this filter ensures exceptional channel isolation in multi-color near-infrared imaging systems. The optimized 1.1mm substrate thickness provides an excellent balance between compact integration and mechanical stability, making it suitable for advanced microscopy, flow cytometry, and in vivo imaging applications. Manufactured by OPTOStokes using advanced high precision coating technology, this filter delivers the precision required for high-end biomedical research and clinical instrumentation.
Technical Specifications
| Parameter | Specification |
|---|---|
| Center Wavelength (CWL) | 720nm at T greater than 90% |
| Transmission Range | 716-723nm at T greater than 90% |
| Full Width at Half Maximum (FWHM) | 10nm ± 2nm |
| Blocking Range | 350-1100nm at T less than 1% |
| Optical Density (OD) | Greater than 2.0 (equivalent to T less than 1%) |
| Substrate Thickness | 1.1mm |
| Surface Quality | 60/40 scratch-dig per MIL-PRF-13830B |
| Coating Type | Hard ion-assisted deposition |
| Part Number | 20218184 |
Key Performance Features
This NIR filter achieves exceptional spectral performance through its narrow 10nm bandwidth design. The tight FWHM provides superior wavelength discrimination, enabling precise isolation of near-infrared fluorophore emissions and effective separation of closely spaced spectral features in multi-channel NIR imaging. This level of spectral precision is essential for applications requiring clean channel separation in the crowded 700-750nm NIR spectral region.
The precisely controlled transmission window spanning 716-723nm represents a narrow passband optimized for Alexa Fluor 700 (emission maximum at 723nm). Peak transmission exceeding 90% within this restrictive window ensures efficient signal collection despite the narrow bandwidth. This combination is achieved through sophisticated thin film coatings design and precision deposition control, resulting in excellent signal-to-noise ratios for NIR fluorescence applications.
The 720nm spectral region offers significant advantages for biological imaging including minimal tissue autofluorescence, excellent tissue penetration depth, reduced light scattering compared to visible wavelengths, and low phototoxicity enabling extended live-cell and in vivo observations. The narrow 10nm bandwidth maximizes spectral purity while collecting sufficient photons for sensitive detection, making it ideal for applications where both wavelength precision and signal strength are critical.
Comprehensive blocking performance with transmission below 1% (optical density greater than 2) from 350-1100nm effectively suppresses visible wavelength crosstalk, completely rejects deep red channels at 650-690nm, blocks excitation light from typical NIR laser sources, and eliminates far-infrared detector noise. This broad rejection ensures clean fluorescence detection even in spectrally demanding multi-parameter systems.
The optimized 1.1mm substrate thickness represents a balanced design, providing superior rigidity compared to 1.0mm substrates while maintaining more compact dimensions than 2.0mm alternatives. This facilitates integration into filter wheels and motorized changers while ensuring adequate mechanical stability and improved thermal performance.
Applications
The BP720-10nm-1.1mm filter excels in near-infrared fluorescence applications demanding precise spectral control:
Multi-Channel NIR Microscopy: Advanced fluorescence imaging systems utilizing 4-6 NIR channels require narrow filters to prevent spectral overlap. The BP720 filter enables precise detection of Alexa Fluor 700 while maintaining complete separation from shorter wavelength NIR fluorophores at 680-700nm and longer wavelength dyes at 750-800nm. Super-resolution microscopy and multi-parameter cell biology studies benefit from the exceptional spectral purity this filter provides.
In Vivo and Deep Tissue Imaging: Whole-animal imaging and deep tissue microscopy leverage NIR wavelengths for maximum tissue penetration. The BP720 filter captures Alexa Fluor 700 emissions with minimal background interference from tissue autofluorescence. The narrow bandwidth enables multi-channel NIR imaging in live animals for tumor detection, biodistribution studies, and therapeutic monitoring. The 720nm region provides optimal balance between fluorophore availability and tissue transparency.
Flow Cytometry: High-parameter flow cytometry panels incorporating multiple NIR fluorophores require filters with excellent spectral separation. The 10nm bandwidth provides the precision necessary to distinguish Alexa Fluor 700 from adjacent NIR dyes, minimizing compensation requirements and improving quantitative accuracy. Clinical diagnostics and immunophenotyping applications benefit from the additional NIR detection capabilities.
Confocal and Multiphoton Microscopy: Laser scanning systems using NIR excitation benefit from the narrow bandwidth for efficient emission collection while completely blocking scattered laser light. The filter enables high-contrast NIR imaging in thick tissue specimens and live animals. Two-photon microscopy applications leverage the deep tissue penetration and reduced scattering at NIR wavelengths.
Western Blot and Protein Quantification: NIR fluorescence detection systems for Western blot applications benefit from the narrow bandwidth and high transmission. The BP720 filter enables detection of Alexa Fluor 700 conjugated secondary antibodies with excellent sensitivity and linear dynamic range, providing accurate protein quantification in multiplexed assays.
Customization and Service
OPTOStokes provides comprehensive customization for NIR filter applications. Center wavelength can be precisely tuned across the 715-725nm range to match specific fluorophore emission maxima. FWHM can be optimized from 8nm for ultimate spectral resolution to 12nm for increased light collection. Enhanced blocking specifications can be implemented in critical spectral regions including visible and far-NIR zones.
Geometric options include circular formats from 12.5mm to 50mm diameter, plus square, rectangular, and custom bandpass shapes. The 1.1mm thickness can be maintained or adjusted to 1.0mm for maximum compactness or 1.5mm/2.0mm for enhanced rigidity. Mounting solutions range from unmounted filters to pre-mounted configurations in threaded rings and filter cubes.
Quality and Availability
Every BP720-10nm-1.1mm filter undergoes rigorous spectral verification using NIR-optimized spectrophotometers with sub-nanometer resolution. Full transmission curves document center wavelength accuracy, FWHM consistency, peak transmission, and blocking performance across 350-1100nm. Surface quality inspection and dimensional measurements ensure specification compliance. OPTOStokes maintains ISO 9001:2015 certification for consistent quality.
Stock availability enables rapid delivery for common configurations. Custom specifications typically ship within 3-4 weeks. Volume production capabilities support OEM requirements with guaranteed batch-to-batch consistency for multi-year programs.
Contact Us
The BP720-10nm-1.1mm filter exemplifies OPTOStokes' expertise in precision near-infrared optical filters. Contact sales@optofilters.com to discuss your NIR imaging requirements, request detailed specifications, or obtain pricing. Visit www.optofilters.com for complete technical resources and product information.
