BP750nm Bandpass Filter | FWHM 10nm, Thickness 1.1mm
The BP750-10nm-1.1mm bandpass filter is precision-engineered for near-infrared fluorescence applications in the optimal tissue transparency window. This advanced bandpass filter features a narrow 10nm full width at half maximum (FWHM) centered at 750nm with transmission greater than 90% across the 746-752nm passband, providing superior wavelength discrimination for Alexa Fluor 750, Cy7, and other near-infrared emitting fluorophores. With comprehensive blocking from 350-1100nm at transmission less than 1%, this filter ensures exceptional channel isolation in multi-color near-infrared imaging systems. The optimized 1.1mm substrate thickness provides an excellent balance between compact integration and mechanical stability. Manufactured by OPTOStokes using advanced high precision coating technology, this filter delivers the precision required for deep tissue imaging, in vivo diagnostics, and advanced biomedical research.
Technical Specifications
| Parameter | Specification |
|---|---|
| Center Wavelength (CWL) | 750nm at T greater than 90% |
| Transmission Range | 746-752nm at T greater than 90% |
| Full Width at Half Maximum (FWHM) | 10nm ± 2nm |
| Blocking Range | 350-1100nm at T less than 1% |
| Optical Density (OD) | Greater than 2.0 (equivalent to T less than 1%) |
| Substrate Thickness | 1.1mm |
| Surface Quality | 60/40 scratch-dig per MIL-PRF-13830B |
| Coating Type | Hard ion-assisted deposition |
| Part Number | 20218101 |
Key Performance Features
This NIR filter achieves exceptional spectral performance in the optimal near-infrared tissue transparency window. The narrow 10nm bandwidth provides superior wavelength discrimination, enabling precise isolation of NIR fluorophore emissions and effective separation of closely spaced channels in multi-parameter imaging. The 750nm spectral region represents the peak of the near-infrared optical window where biological tissue exhibits maximum transparency, minimal absorption, and reduced scattering.
The precisely controlled transmission window spanning 746-752nm represents a narrow passband optimized for Alexa Fluor 750 (emission maximum at 775nm with significant emission in the 750nm region), Cy7, and IRDye 800CW. Peak transmission exceeding 90% within this restrictive window ensures efficient signal collection despite the narrow bandwidth. This combination is achieved through sophisticated thin film coatings design and precision deposition control.
The 750nm wavelength offers critical advantages for biological imaging including maximum tissue penetration depth, minimal hemoglobin and water absorption, virtually absent tissue autofluorescence, and excellent signal-to-background ratios in live animal imaging. The narrow 10nm bandwidth maximizes spectral purity while collecting sufficient photons for sensitive fluorescence detection, making it ideal for applications demanding both wavelength precision and deep tissue penetration.
Comprehensive blocking performance with transmission below 1% (optical density greater than 2) from 350-1100nm effectively suppresses all visible wavelength channels, completely rejects shorter wavelength NIR channels at 650-730nm, and eliminates detector noise from longer wavelengths. This broad rejection ensures clean signal detection even in challenging in vivo imaging scenarios with high background levels.
The optimized 1.1mm substrate thickness provides superior mechanical stability compared to thinner alternatives while maintaining compact dimensions for integration into filter wheels and imaging systems. The balanced design ensures reliable performance in demanding research and clinical environments.
Applications
The BP750-10nm-1.1mm filter excels in near-infrared fluorescence applications leveraging the optimal tissue transparency window:
In Vivo Whole-Animal Imaging: Small animal imaging systems for preclinical research utilize the 750nm window for maximum tissue penetration. The BP750 filter captures Alexa Fluor 750 and Cy7 emissions with minimal tissue absorption and scattering, enabling deep tissue tumor detection, biodistribution studies, and therapeutic monitoring. The narrow bandwidth enables multi-channel NIR imaging for tracking multiple targets simultaneously in living animals.
Deep Tissue Microscopy: Intravital microscopy and thick tissue imaging applications benefit from the superior tissue penetration at 750nm. The filter enables visualization of fluorescently labeled structures several millimeters deep in tissue, far exceeding the penetration depth achievable with visible wavelengths. Applications include tumor microenvironment studies, neuroscience imaging, and developmental biology research in intact organisms.
Surgical Guidance and Intraoperative Imaging: Real-time fluorescence-guided surgery systems use NIR fluorophores in the 750nm region to visualize tumor margins, lymph nodes, and vascular structures during surgical procedures. The BP750 filter provides precise spectral separation enabling simultaneous detection of multiple NIR markers for comprehensive surgical navigation.
Multi-Channel NIR Flow Cytometry: Advanced flow cytometry panels incorporating 6-8 NIR fluorophores require narrow filters for effective channel separation. The 10nm bandwidth provides the precision necessary to distinguish Alexa Fluor 750 from adjacent NIR dyes at 700-730nm and 780-800nm, enabling high-parameter immunophenotyping with minimal spectral overlap.
NIR Western Blot and Imaging: Protein detection systems utilizing NIR fluorescence benefit from the 750nm region's low background interference. The BP750 filter enables sensitive detection of Alexa Fluor 750 and IRDye 800 conjugated antibodies with excellent linearity and dynamic range for accurate protein quantification in complex samples.
Customization and Service
OPTOStokes provides comprehensive customization for NIR filter applications in the tissue transparency window. Center wavelength can be precisely tuned across the 745-760nm range to match specific fluorophore emission characteristics. FWHM can be optimized from 8nm for ultimate spectral resolution to 12nm for increased light collection. Enhanced blocking specifications can be implemented in critical spectral regions.
Geometric options include circular formats from 12.5mm to 50mm diameter, plus square, rectangular, and custom bandpass shapes. The 1.1mm thickness can be maintained or adjusted to 1.0mm for maximum compactness or 1.5mm/2.0mm for enhanced rigidity. Mounting solutions range from unmounted filters to pre-mounted configurations in threaded rings and filter cubes.
Quality and Availability
Every BP750-10nm-1.1mm filter undergoes rigorous spectral verification using NIR-optimized spectrophotometers with sub-nanometer resolution. Full transmission curves document center wavelength accuracy, FWHM consistency, peak transmission, and blocking performance across the full UV-NIR range. Surface quality inspection and dimensional measurements ensure specification compliance. OPTOStokes maintains ISO 9001:2015 certification for consistent quality.
Stock availability enables rapid delivery for common configurations. Custom specifications typically ship within 3-4 weeks. Volume production capabilities support OEM requirements with guaranteed batch-to-batch consistency for long-term programs.
Contact Us
The BP750-10nm-1.1mm filter exemplifies OPTOStokes' expertise in precision near-infrared optical filters for the tissue transparency window. Contact sales@optofilters.com to discuss your deep tissue imaging requirements, request detailed specifications, or obtain pricing. Visit www.optofilters.com for complete technical resources and product information.
