BP660nm Bandpass Filter | FWHM 80nm, Thickness 1.0mm
The BP660-80nm-1.0mm bandpass filter is engineered for applications demanding maximum light collection efficiency in the deep red spectral region. Featuring an exceptionally wide 80nm full width at half maximum (FWHM) centered at 660nm, this bandpass filter delivers superior photon throughput for fluorescence detection of Cy5, Alexa Fluor 647, APC, and other deep red emitting fluorophores. With a broad transmission window spanning 630-695nm at transmission greater than 90% and comprehensive blocking from 400-1100nm at transmission less than 1%, this filter maximizes signal strength while maintaining effective spectral isolation. The compact 1.0mm substrate thickness enables integration into space-constrained optical assemblies while reducing optical path length and focal shift. Manufactured by OPTOStokes using advanced high precision coating technology, this filter delivers exceptional performance for high-throughput screening, rapid flow cytometry, and photon-limited detection applications.
Technical Specifications
| Parameter | Specification |
|---|---|
| Center Wavelength (CWL) | 660nm ± 5nm at T greater than 90% |
| Transmission Range | 630-695nm at T greater than 90% |
| Full Width at Half Maximum (FWHM) | 80nm ± 5nm |
| Blocking Range | 400-1100nm at T less than 1% |
| Optical Density (OD) | Greater than 2.0 (equivalent to T less than 1%) |
| Substrate Thickness | 1.0mm |
| Surface Quality | 60/40 scratch-dig per MIL-PRF-13830B |
| Coating Type | Hard ion-assisted deposition |
| Part Number | 20220401 |
Key Performance Features
This wide FWHM bandpass filter demonstrates exceptional light collection characteristics optimized for deep red fluorescence applications. The ultra-wide 80nm FWHM provides dramatically greater photon collection compared to narrow 10-30nm bandpass alternatives, making it ideal for photon-starved detection scenarios, high-speed imaging, and maximum signal throughput applications. Peak transmission exceeding 90% across the extensive 630-695nm range ensures maximum photon capture, critical for time-resolved measurements, high-frame-rate microscopy, and rapid flow cytometry.
The exceptionally broad passband efficiently captures the full emission spectrum of multiple deep red fluorophores simultaneously. Cy5 with emission maximum at 670nm, Alexa Fluor 647 centered at 665nm, APC (Allophycocyanin) with emission spanning 660-680nm, and Alexa Fluor 680 all fall within the filter's high-transmission window. This comprehensive spectral coverage maximizes detected signal levels, dramatically improves signal-to-noise ratios, and enables shorter exposure times in fluorescence detection applications.
Despite the ultra-wide passband designed for maximum light collection, the filter maintains effective out-of-band blocking with transmission below 1% (optical density greater than 2) from 400-1100nm. This comprehensive rejection suppresses orange-red channel crosstalk from 590-620nm fluorophores, blocks excitation light from typical 633nm and 640nm laser sources, and eliminates near-infrared interference from 700-1100nm. The deep blocking enables clean signal detection even with intense excitation sources.
The compact 1.0mm substrate thickness delivers critical advantages for modern optical instrument design. The minimal thickness reduces optical path length, virtually eliminates focal shift in tightly designed systems, and enables high-density filter packing in automated filter wheels. The reduced mass facilitates faster switching speeds and minimizes mechanical complexity in motorized filter changers.
Applications
The BP660-80nm-1.0mm filter excels across applications where maximum deep red light collection and rapid detection are essential:
High-Throughput Flow Cytometry: Rapid cell analysis systems processing thousands of events per second require maximum fluorescence collection efficiency. The BP660 filter captures maximum emission from APC, Alexa Fluor 647, and Cy5-conjugated antibodies used in immunophenotyping panels. The ultra-wide bandwidth accommodates spectral variation between fluorophore lots and temperature-induced shifts during high-speed operation. Clinical diagnostic applications benefit from the enhanced sensitivity enabling detection of dim markers and rare cell populations.
High-Content Screening and Drug Discovery: Automated imaging platforms analyzing thousands of wells require rapid fluorescence detection with maximum signal collection. The 80nm bandwidth maximizes signal strength from deep red fluorescent proteins and dyes, enabling shorter exposure times and higher throughput. Pharmaceutical research applications including compound screening and cellular phenotyping rely on this performance for accelerated screening cycles.
Widefield Fluorescence Microscopy: Traditional widefield systems prioritize imaging speed and field of view over spectral resolution, making ultra-wide bandpass filters ideal. The BP660 filter efficiently captures emissions from Cy5, Alexa Fluor 647, and other deep red fluorophores in multi-color imaging experiments. The wide bandwidth enables lower excitation power and shorter exposure times, reducing photobleaching and phototoxicity in live-cell imaging applications.
Rapid Flow Cytometry and Cell Sorting: High-speed cell sorting applications require efficient fluorescence collection at particle velocities exceeding 10,000 events per second. The ultra-wide passband maximizes photon collection during brief detection windows, improving sort accuracy and purity. Rare cell enrichment and cell line development applications benefit from the enhanced detection sensitivity.
Time-Lapse and Live-Cell Imaging: Long-term imaging experiments monitoring cellular processes require filters that maximize signal collection while minimizing phototoxicity. The 80nm bandwidth enables extremely low excitation power and minimal exposure times, dramatically reducing cellular stress during extended imaging sessions. Developmental biology and cellular dynamics studies benefit from improved cell viability and extended observation periods.
Photon-Limited Detection: Applications involving weak fluorescence signals, thick tissue specimens, or small sample volumes benefit from the maximum light collection this filter provides. The wide bandwidth captures significantly more photons than narrow alternatives, improving detection limits and enabling measurements that would be impossible with restrictive bandpass filters.
Customization and Service
OPTOStokes provides comprehensive customization options. Center wavelength can be tuned across the 655-665nm range. FWHM can be adjusted from 70nm to 90nm depending on application priorities. Enhanced blocking specifications can be implemented for specific spectral regions. Geometric options include circular, square, and custom bandpass shapes in various sizes.
Mounting solutions range from unmounted filters for direct integration to pre-mounted configurations in threaded rings and filter cubes. Complete filter sets combining excitation, emission, and dichroic components are available. Environmental hardening ensures reliable performance in demanding conditions.
Quality and Availability
Every filter undergoes comprehensive spectral verification using calibrated spectrophotometers, with full transmission curves documented. Quality documentation includes surface quality inspection and dimensional measurements. OPTOStokes maintains ISO 9001:2015 certification ensuring consistent quality. Stock availability enables rapid delivery, with most orders shipping within 24-48 hours.
Contact Us
The BP660-80nm-1.0mm filter represents OPTOStokes' commitment to high-performance optical solutions. Contact sales@optofilters.com to discuss requirements, request specifications, or obtain pricing. Visit www.optofilters.com for complete technical resources and product information.
