BP780nm Bandpass Filter | FWHM 23nm, Thickness 2.0mm
The BP780-23nm-2.0mm bandpass filter is engineered for near-infrared fluorescence applications demanding optimal performance in the tissue transparency window. This versatile bandpass filter features a 23nm full width at half maximum (FWHM) centered at 780nm with transmission greater than 90% across the 775-790nm passband, providing ideal performance for Cy7, IRDye 800CW, and other near-infrared emitting fluorophores. With comprehensive blocking from 350-1100nm at transmission less than 1%, this filter ensures clean signal isolation in multi-channel near-infrared imaging systems. The robust 2.0mm substrate thickness provides enhanced mechanical stability, superior thermal management, and high laser damage threshold for demanding applications. Manufactured by OPTOStokes using advanced high precision coating technology, this filter delivers consistent performance for deep tissue imaging, in vivo diagnostics, and biomedical research applications.
Technical Specifications
| Parameter | Specification |
|---|---|
| Center Wavelength (CWL) | 780nm ± 3nm at T greater than 90% |
| Transmission Range | 775-790nm at T greater than 90% |
| Full Width at Half Maximum (FWHM) | 23nm ± 3nm |
| Blocking Range | 350-1100nm at T less than 1% |
| Optical Density (OD) | Greater than 2.0 (equivalent to T less than 1%) |
| Substrate Thickness | 2.0mm |
| Surface Quality | 60/40 scratch-dig per MIL-PRF-13830B |
| Coating Type | Hard ion-assisted deposition |
| Part Number | 20221313 |
Key Performance Features
This NIR filter achieves excellent spectral performance through its balanced 23nm bandwidth design. The FWHM provides an optimal balance between wavelength selectivity and light collection efficiency, making it suitable for applications where both spectral purity and signal strength are important. Peak transmission exceeding 90% across the 775-790nm range ensures maximum photon throughput while maintaining adequate separation from shorter wavelength NIR channels at 700-760nm and longer wavelength channels at 800-850nm.
The precisely controlled passband efficiently captures emissions from Cy7 (emission maximum at 767nm with significant emission extending into the 775-790nm range), IRDye 800CW (emission peak at 794nm), and other NIR fluorophores used in deep tissue and in vivo imaging. The 780nm spectral region represents a critical point in the near-infrared optical window where biological tissue exhibits excellent transparency, minimal absorption from hemoglobin and water, and virtually absent autofluorescence. The 23nm bandwidth collects sufficient fluorophore emission to maximize signal-to-noise ratios while preventing excessive spectral overlap with adjacent channels.
Comprehensive blocking performance with transmission below 1% (optical density greater than 2) from 350-1100nm effectively suppresses all visible wavelength channels, completely rejects shorter NIR channels, and eliminates far-infrared detector noise. This deep rejection ensures clean fluorescence detection even in challenging in vivo imaging scenarios with complex spectral backgrounds.
The robust 2.0mm substrate thickness provides enhanced mechanical stability compared to thinner alternatives, facilitating secure mounting in precision optical assemblies. The increased thickness improves thermal dissipation, reducing temperature-induced wavelength shifts during extended operation or high-power laser applications. The substantial substrate also ensures high laser damage threshold, making this filter suitable for intense NIR laser systems used in advanced imaging modalities.
Applications
The BP780-23nm-2.0mm filter excels across diverse near-infrared fluorescence applications in the optimal tissue window:
In Vivo Whole-Animal Imaging: Preclinical imaging systems for small animal research utilize the 780nm window for deep tissue penetration and low background imaging. The BP780 filter captures Cy7 and IRDye 800CW emissions with excellent sensitivity, enabling tumor detection, biodistribution studies, and therapeutic response monitoring in living animals. The balanced bandwidth maximizes signal collection while maintaining adequate spectral resolution for multi-channel NIR imaging of multiple biomarkers simultaneously.
Deep Tissue and Intravital Microscopy: Advanced microscopy techniques imaging several millimeters deep in tissue benefit from the superior tissue transparency at 780nm. The filter enables visualization of fluorescently labeled cells, blood vessels, and molecular targets in intact organs and thick tissue specimens. Applications include tumor microenvironment imaging, neuroscience studies, and developmental biology research where deep penetration is essential.
Fluorescence-Guided Surgery: Intraoperative imaging systems use NIR fluorophores in the 780nm region to provide real-time surgical guidance. The BP780 filter enables visualization of tumor margins, sentinel lymph nodes, and vascular structures during surgical procedures. The robust 2.0mm substrate ensures reliable performance in demanding clinical environments.
Multi-Channel Flow Cytometry: Advanced flow cytometry panels incorporating multiple NIR fluorophores benefit from the balanced spectral characteristics. The 23nm bandwidth provides adequate channel separation while collecting sufficient fluorescence for sensitive detection at high cell velocities. Clinical diagnostic applications including immunophenotyping and cell subset analysis leverage the additional NIR detection capabilities.
NIR Fluorescence Microscopy: Confocal and widefield microscopy systems utilizing NIR fluorophores benefit from reduced phototoxicity and photobleaching compared to visible wavelengths. The BP780 filter enables clean detection of Cy7, IRDye conjugates, and NIR fluorescent proteins in multi-color imaging experiments. The 2.0mm thickness withstands high laser power densities in laser scanning applications.
Customization and Service
OPTOStokes provides comprehensive customization options to optimize filter performance for specific NIR applications. Center wavelength can be tuned across the 775-785nm range to match specific fluorophore emission characteristics. FWHM can be adjusted from 20nm for enhanced spectral resolution to 30nm for increased light collection. Enhanced blocking specifications can be implemented in critical spectral regions.
Geometric options include circular formats from 12.5mm to 50mm diameter, plus square, rectangular, and custom bandpass shapes. The 2.0mm thickness can be maintained for maximum stability or adjusted to 1.0mm/1.5mm for more compact systems. Mounting solutions range from unmounted filters to pre-mounted configurations in threaded rings and filter cubes. Complete NIR filter sets are available.
Quality and Availability
Every BP780-23nm-2.0mm filter undergoes comprehensive spectral verification using NIR-optimized spectrophotometers, with full transmission curves from 350-1100nm documented. Surface quality inspection and dimensional measurements ensure specification compliance. OPTOStokes maintains ISO 9001:2015 certification ensuring consistent quality across all production volumes.
Stock availability enables rapid delivery for common configurations, with most orders shipping within 24-48 hours. Custom specifications typically deliver within 3-4 weeks. Volume production capabilities support OEM requirements with guaranteed batch-to-batch consistency.
Contact Us
The BP780-23nm-2.0mm filter represents OPTOStokes' commitment to delivering high-performance near-infrared optical solutions. Contact sales@optofilters.com to discuss your deep tissue imaging requirements, request detailed specifications, or obtain pricing. Visit www.optofilters.com for complete technical resources and product information.
