BP605nm Bandpass Filter | FWHM 46nm, Thickness 1.1mm
The BP605-46nm-1.1mm bandpass filter is engineered for applications demanding maximum light collection in the orange-red spectral region. Featuring a wide 46nm full width at half maximum (FWHM) centered at 605nm, this bandpass filter delivers exceptional photon throughput for fluorescence detection of mCherry, Texas Red, PE-Cy5, and other orange-red emitting fluorophores. With a broad transmission window spanning 585-620nm at transmission greater than 90% and comprehensive blocking from 350-1100nm at transmission less than 1%, this filter maximizes signal strength while maintaining effective spectral isolation. The optimized 1.1mm substrate thickness provides an ideal balance between compact integration and mechanical stability, making it suitable for diverse optical system architectures. Manufactured by OPTOStokes using advanced high precision coating technology, this filter delivers consistent performance for demanding research and clinical applications.
Technical Specifications
| Parameter | Specification |
|---|---|
| Center Wavelength (CWL) | 605nm ± 5nm at T greater than 90% |
| Transmission Range | 585-620nm at T greater than 90% |
| Full Width at Half Maximum (FWHM) | 46nm ± 3nm |
| Blocking Range | 350-1100nm at T less than 1% |
| Optical Density (OD) | Greater than 2.0 (equivalent to T less than 1%) |
| Substrate Thickness | 1.1mm |
| Surface Quality | 60/40 scratch-dig per MIL-PRF-13830B |
| Coating Type | Hard ion-assisted deposition |
| Part Number | 20209090 |
Key Performance Features
This wide 660nm bandpass region filter demonstrates performance characteristics optimized for orange-red fluorescence applications requiring maximum signal collection. The wide 46nm FWHM provides substantially greater light gathering capability compared to narrow 15-25nm bandpass alternatives, making it ideal for photon-limited detection scenarios, high-speed imaging, and weak fluorescence measurements. Peak transmission exceeding 90% across the broad 585-620nm range ensures maximum photon throughput, critical for time-resolved measurements and high-frame-rate microscopy.
The wide passband efficiently captures the full emission spectrum of commonly used orange-red fluorophores. mCherry with emission maximum at 610nm, Texas Red centered at 615nm, PE-Cy5 with emission spanning 660-680nm (with significant emission extending into the 600-620nm range), and DsRed derivatives all benefit from this broad spectral acceptance. This maximizes detected signal levels, improves signal-to-noise ratios, and reduces exposure times in fluorescence detection applications.
Despite the wide passband designed for maximum light collection, the filter maintains effective out-of-band blocking with transmission below 1% (optical density greater than 2) from 350-1100nm. This comprehensive rejection suppresses yellow-green channel crosstalk from 530-580nm fluorophores, blocks excitation light from typical 555-590nm sources, and eliminates far-red and near-infrared interference from 650-1100nm. The deep blocking enables clean signal detection in multi-channel imaging systems.
The optimized 1.1mm substrate thickness represents a balanced design choice, providing greater rigidity than ultra-thin 1.0mm substrates while maintaining more compact dimensions than standard 2.0mm alternatives. This intermediate thickness facilitates integration into space-constrained optical assemblies while ensuring adequate mechanical stability and improved thermal dissipation compared to thinner substrates.
Applications
The BP605-46nm-1.1mm filter excels across diverse applications where maximum orange-red light collection and compact integration are essential:
Widefield Fluorescence Microscopy: Traditional widefield systems prioritize imaging speed and field of view, making wide bandpass filters ideal for efficient emission collection. The BP605 filter efficiently captures emissions from mCherry, tdTomato, Texas Red, and DsRed fluorophores commonly used in cellular imaging, protein localization, and tissue histology. The wide bandwidth maximizes signal collection enabling shorter exposure times and reduced photobleaching in live-cell imaging.
Flow Cytometry and Cell Analysis: Flow cytometry systems analyzing cells at high velocities require efficient fluorescence collection. The BP605 filter captures maximum emission from PE-Texas Red, PE-Cy5, and tandem conjugates used in immunophenotyping panels. The wide passband accommodates spectral variation between fluorophore lots and temperature-induced shifts. Clinical diagnostic applications rely on this filter's high collection efficiency for accurate cell quantification.
High-Throughput Screening: Automated microplate readers and high-content imaging systems require rapid, reliable fluorescence detection across multi-well formats. The BP605 filter enables efficient detection of orange-red fluorescent proteins and dyes used in compound screening and cellular phenotyping. The wide bandwidth maximizes signal strength, improving assay sensitivity and dynamic range.
Confocal Microscopy: Point-scanning confocal systems employ bandpass filters to collect fluorescence while rejecting laser scatter. The BP605 filter efficiently captures mCherry and Texas Red emissions following 561nm or 594nm laser excitation. The wide bandwidth collects more of the fluorophore emission spectrum, improving signal levels in deep tissue imaging and thick specimen applications.
Live-Cell Imaging and Time-Lapse Microscopy: Long-term imaging experiments monitoring cellular processes require filters that maximize signal collection while minimizing phototoxicity. The wide FWHM bandpass characteristics enable lower excitation power and shorter exposure times, reducing cellular stress and improving viability during extended imaging sessions.
Customization and Service
OPTOStokes provides comprehensive customization options. Center wavelength can be tuned across the 600-610nm range. FWHM can be adjusted from 40nm to 55nm depending on application priorities. Enhanced blocking specifications can be implemented for specific spectral regions. Geometric options include circular, square, and custom bandpass shapes in various sizes.
Mounting solutions range from unmounted filters for direct integration to pre-mounted configurations in threaded rings and filter cubes. Multi-filter assemblies combining this emission filter with complementary excitation filters and dichroic beamsplitters are available as integrated modules. Environmental hardening ensures reliable performance in demanding conditions.
Quality and Availability
Every filter undergoes comprehensive spectral verification using calibrated spectrophotometers, with full transmission curves documented. Quality documentation includes surface
