This Cy3 fluorescence filter set delivers excellent signal-to-noise, outstanding blocking, and superior image contrast for advanced fluorescence microscopy.
When installed in your fluorescence microscope, these filters significantly enhance image clarity and brightness. The high transmittance in the passband ensures maximum imaging brightness, while deep blocking in the stopband keeps unwanted spectral cross-talk away from the detector.
Compared with conventional stepped filters, this set achieves a much higher signal-to-noise ratio — ensuring that every detail stands out, both for science and diagnostics. Suitably designed for all mainstream Cy3 fluorescence protocols, it is also compatible with common multi-color, immunofluorescence, and cell biology applications.
Kit Components & Technical Specifications
- Excitation Bandpass Filter (BP543-20nm-1.0mm)
| Center Wavelength (CWL) | 543nm ± 2nm at T greater than 90% |
| Transmission Range | 538–548nm, average T greater than 90% |
| Bandwidth (FWHM) | 20nm ± 2nm |
| Blocking Ranges / OD Levels | 350–523nm at average OD greater than 6 (minimum OD greater than 5); 563–800nm at average OD greater than 6 (minimum OD greater than 5); 800–1050nm at OD greater than 3 |
| Angle of Incidence (AOI) | 0° |
| Thickness | 1.0mm |
- Emission Bandpass Filter (BP585-20nm-1.0mm)
| Center Wavelength (CWL) | 585nm ± 2nm at T greater than 90% |
| Transmission Range | 580–590nm, average T greater than 90% |
| Bandwidth (FWHM) | 20nm ± 2nm |
| Blocking Ranges / OD Levels | 350–565nm at average OD greater than 6 (minimum OD greater than 5); 602.5–800nm at average OD greater than 6 (minimum OD greater than 5); 800–1050nm at OD greater than 3 |
| Angle of Incidence (AOI) | 0° |
| Thickness | 1.0mm |
- Dichroic Beamsplitter (DM565-1.0mm)
| Transmission Range | 575–700nm at T greater than 95% |
| Blocking Range | 430–555nm at T less than 2% |
| Angle of Incidence (AOI) | 45° |
| Thickness | 1.0mm |
Key Benefits
- Exceptional image clarity for Cy3, TRITC, and related dyes – designed for modern fluorescence and multi-color labs
- High signal transmission (T > 90%) ensures bright, high-contrast fluorescence output
- Deep out-of-band blocking (OD > 6) eliminates cross-talk and unwanted background
- Faster, sharper imaging – balanced spectral performance over conventional filter sets
- Compatible with all major fluorescence microscopes and microscopy platforms (upright, inverted, confocal)
Typical Applications
- Cy3, TRITC and Alexa Fluor 555 fluorescence labeling
- Immunofluorescence and FISH imaging
- Cell biology, molecular diagnostics, neuroscience imaging
- Multi-color and live cell fluorescence microscopy
Custom & Support
OPTOStokes provides rapid customization (diameter, thickness, mounting ring, assembly), tech consultations, and fast-response OEM delivery for research or instrumentation. For spec drawings, datasheets, or expert support, contact sales@optofilters.com or visit www.optofilters.com.
