The DAPI-BP460-55nm-OD6 bandpass filter delivers exceptional blue-channel fluorescence control for DAPI, Hoechst, and blue-excited fluorophores. With a 55nm full width at half maximum (FWHM) centered at 460nm and average transmission greater than 90% across the 440-480nm window, it ensures unmatched signal strength for cell and tissue nuclear staining.
Technical Specifications
| Parameter | Specification |
|---|---|
| Center Wavelength (CWL) | 460nm ± 2nm |
| Transmission Range | 440-480nm at average T greater than 90% |
| Full Width at Half Maximum (FWHM) | 55nm ± 2nm |
| Blocking Range | 200-420nm at average OD greater than 6 (minimum OD greater than 5); 520-700nm at average OD greater than 6 (minimum OD greater than 5); 700-1000nm at OD greater than 3 |
| Blocking Depth | OD6 (deep blocking), multi-zone controlled |
| Incident Angle | 0° |
| Coating | Dual-side hard ion-assisted deposition |
| Reference | DAPI-BP460-55nm-OD6 |
Key Performance Features
This bandpass filter sets the benchmark for DAPI and blue channel imaging—combining OD6 deep blocking outside the 440-480nm transmission range with dual-side coatings for maximum crosstalk suppression in advanced multi-channel fluorescence microscopy.
Average T greater than 90% ensures robust DAPI, Hoechst, or blue dye signal push-through for high-magnification, low-background imaging. OD6 blocking at UV and visible red/far-red zones provides negligible bleed-through, even when exposed to broad-spectrum or high-intensity illumination sources.
Applications
- DAPI/Hoechst nuclear fluorescence imaging: Immunofluorescence, genetic and cell biology, pathology, developmental studies. - High-parameter multi-channel microscopy: OD6 levels eliminate crosstalk in advanced 4-7 color setups. - Laser and LED widefield/confocal platforms: Robust for high SNR, low autofluorescence, and artifact-free scanning. - Diagnostics and automation: Consistent quality for cytometry, histology, and high-throughput plate readers.
Customization & Service
OPTOStokes delivers standard and custom specs: size, shape, and mounting. ISO 9001:2015, data-driven QC, fast prototyping for OEM, research, or clinical use. Datasheets and technical help—email sales@optofilters.com or visit www.optofilters.com.
Pain Points & CTA
Difficulty with blue channel crosstalk or signal loss? Switch to BP460-55nm-OD6 for OD6 blocking, dual-surface coatings, and ultra-high consistency. Contact us for tech solutions, samples, or integration guidelines.
