FAQ: How to Choose the Right Filters for Cy5 Fluorophores?

Selecting optimal filters for Cy5 fluorescence imaging requires matching the fluorophore’s spectral properties with precision. At OPTOStokes, our specialized Cy5 filter sets are engineered to enhance signal clarity, ensuring reliable results in microscopy, flow cytometry, and other imaging applications.

Key Wavelength Parameters for Cy5 Filters

Cy5 fluorophores have distinct excitation and emission profiles, requiring filter specifications tailored to these ranges:

• Excitation Filter: 630/20nm (centered at 630nm with 20nm bandwidth) – captures the peak excitation wavelength of Cy5.
• Emission Filter: 675/20nm (centered at 675nm with 20nm bandwidth) – isolates Cy5’s emission signal while blocking background noise.
• Dichroic Mirror: DM647nm – efficiently reflects excitation light and transmits emission light, minimizing crosstalk.

Filter Types for Optimal Cy5 Performance

To maximize imaging quality, consider the following filter types:

• Bandpass Filters: Ideal for isolating specific wavelength ranges (e.g., 630/20nm excitation), ensuring high signal-to-noise ratio (SNR).
• Dichroic Filters (Beam Splitters): Critical for separating excitation and emission light paths, preventing excitation light from reaching the detector.

OPTOStokes’ Cy5 filter sets are designed to be compatible with most imaging systems, from confocal microscopes to high-throughput plate readers.

Why Choose OPTOStokes for Cy5 Filters?

Our Cy5 filters balance precision and practicality:

• Ready-to-Ship Options: Standard 630/20nm, 675/20nm, and DM647nm filters are in stock for immediate delivery.
• Custom Solutions: Need adjusted bandwidths (e.g., 635/15nm) or system-specific compatibility? Our team designs tailored filters to match your setup.
• Global Quality: All filters meet international standards for wavelength accuracy and durability, ensuring consistent performance across batches.

Get Started with Your Cy5 Filter Set

Not sure which Cy5 filter configuration fits your imaging system? Let our experts help:

• Email sales@optofilters.com for:    - Detailed spec sheets for 630/20nm excitation filters    - Custom filter design consultations
• Visit our website to:    - Browse in-stock Cy5 filter sets    - Request a quote within 24 hours

OPTOStokes: Your partner for precise, reliable Cy5 fluorescence imaging filters.