BP700nm Bandpass Filter | FWHM 20nm, Thickness 1.1mm
The BP700-20nm-1.1mm bandpass filter is precision-engineered for near-infrared fluorescence applications demanding optimal performance in the 700nm spectral region. This versatile bandpass filter features a 20nm full width at half maximum (FWHM) centered at 700nm with transmission greater than 90% across the 695-705nm passband, providing ideal performance for Alexa Fluor 700, IRDye 680LT, and other near-infrared emitting fluorophores. With comprehensive blocking from 350-1100nm at transmission less than 1%, this filter ensures clean signal isolation in multi-channel near-infrared imaging systems. The optimized 1.1mm substrate thickness provides an excellent balance between compact integration and mechanical stability, making it suitable for diverse microscopy, flow cytometry, and in vivo imaging applications. Manufactured by OPTOStokes using advanced high precision coating technology, this filter delivers consistent performance for demanding biomedical research and clinical applications.
Technical Specifications
| Parameter | Specification |
|---|---|
| Center Wavelength (CWL) | 700nm ± 2nm at T greater than 90% |
| Transmission Range | 695-705nm at T greater than 90% |
| Full Width at Half Maximum (FWHM) | 20nm ± 2nm |
| Blocking Range | 350-1100nm at T less than 1% |
| Optical Density (OD) | Greater than 2.0 (equivalent to T less than 1%) |
| Substrate Thickness | 1.1mm |
| Surface Quality | 60/40 scratch-dig per MIL-PRF-13830B |
| Coating Type | Hard ion-assisted deposition |
| Part Number | 20219588 |
Key Performance Features
This NIR filter achieves exceptional spectral performance in the near-infrared region through its optimized 20nm bandwidth design. The FWHM provides an ideal balance between wavelength selectivity and light collection efficiency, making it suitable for applications where both spectral purity and signal strength are critical. Peak transmission exceeding 90% across the 695-705nm range ensures maximum photon throughput while maintaining adequate separation from deep red channels at 660-680nm and far-NIR channels at 750-800nm.
The precisely controlled passband efficiently captures emissions from Alexa Fluor 700 (emission maximum at 723nm with significant emission extending into the 695-705nm range), IRDye 680LT, and other near-infrared fluorophores used in multi-color imaging. The 700nm spectral region offers significant advantages for biological imaging including reduced tissue autofluorescence, deeper tissue penetration compared to visible wavelengths, and minimal phototoxicity enabling extended live-cell observation. The 20nm bandwidth collects sufficient fluorophore emission to maximize signal-to-noise ratios while preventing excessive spectral overlap with adjacent channels.
Comprehensive blocking performance with transmission below 1% (optical density greater than 2) from 350-1100nm effectively suppresses visible wavelength crosstalk, blocks excitation light from typical 633-690nm laser sources, and eliminates far-infrared interference. This deep rejection ensures clean fluorescence detection in multi-channel imaging systems and prevents detector saturation from intense excitation sources.
The optimized 1.1mm substrate thickness represents a balanced engineering choice, providing superior rigidity compared to ultra-thin 1.0mm substrates while maintaining more compact dimensions than standard 2.0mm alternatives. This intermediate thickness facilitates integration into space-constrained filter wheels and motorized changers while ensuring adequate mechanical stability and improved thermal performance.
Applications
The BP700-20nm-1.1mm filter excels across diverse near-infrared fluorescence applications demanding balanced performance:
In Vivo and Deep Tissue Imaging: Near-infrared wavelengths penetrate biological tissue more effectively than visible light, enabling deeper imaging with reduced scattering. The BP700 filter captures emissions from NIR fluorophores used in whole-animal imaging, deep tissue microscopy, and surgical guidance applications. The reduced tissue autofluorescence at 700nm dramatically improves contrast compared to visible wavelength imaging, enabling visualization of deeper anatomical structures and tumor margins.
Multi-Channel Flow Cytometry: Advanced flow cytometry panels incorporating 8-15 parameters increasingly utilize near-infrared fluorophores to expand spectral capabilities. The BP700 filter enables detection of Alexa Fluor 700 and similar NIR dyes while maintaining separation from deep red APC/Alexa 647 channels at 660-680nm and far-NIR channels at 750-780nm. Clinical diagnostic applications benefit from the additional detection channels for comprehensive immunophenotyping.
NIR Fluorescence Microscopy: Confocal and widefield microscopy systems utilizing near-infrared fluorophores benefit from reduced autofluorescence and photobleaching. The BP700 filter enables clean detection of Alexa Fluor 700, IRDye conjugates, and NIR fluorescent proteins in multi-color imaging experiments. Cell biology research applications including protein trafficking, cellular co-localization, and live-cell dynamics studies leverage the advantages of NIR imaging.
Small Animal Imaging: Preclinical research employing optical imaging in mice and rats utilizes NIR fluorophores for tumor detection, biodistribution studies, and therapeutic monitoring. The 700nm spectral region provides optimal balance between tissue penetration and fluorophore availability. The filter's blocking performance suppresses background tissue autofluorescence, improving detection sensitivity for targeted imaging agents.
Western Blot and Protein Detection: NIR fluorescence detection in Western blot applications offers superior linearity and dynamic range compared to chemiluminescence. The BP700 filter enables detection of IRDye 680 and Alexa Fluor 700 conjugated secondary antibodies, providing quantitative protein analysis with multiplexing capabilities.
Customization and Service
OPTOStokes provides comprehensive customization options to optimize filter performance for specific NIR applications. Center wavelength can be tuned across the 695-710nm range to match specific fluorophore emission maxima. FWHM can be adjusted from 15nm for enhanced spectral resolution to 25nm for increased light collection. Blocking specifications can be enhanced in critical spectral regions including deep red and far-NIR zones.
Geometric options include circular formats from 12.5mm to 50mm diameter, plus square, rectangular, and custom bandpass shapes. The 1.1mm thickness can be maintained or adjusted to 1.0mm for maximum compactness or 1.5mm/2.0mm for enhanced rigidity. Mounting solutions range from unmounted filters to pre-mounted configurations in threaded rings and filter cubes. Complete NIR filter sets are available for simplified microscopy system integration.
Quality and Availability
Every BP700-20nm-1.1mm filter undergoes comprehensive spectral verification using NIR-optimized spectrophotometers, with full transmission curves from 350-1100nm documented in detailed test reports. Surface quality inspection and dimensional metrology ensure specification compliance. OPTOStokes maintains ISO 9001:2015 certification ensuring consistent quality across all production volumes.
Stock availability of common configurations enables rapid delivery, with most orders shipping within 24-48 hours. Custom specifications typically deliver within 3-4 weeks. Volume production capabilities support OEM customers requiring consistent NIR filter supply for multi-year programs.
Contact Us
The BP700-20nm-1.1mm filter represents OPTOStokes' commitment to delivering high-performance near-infrared optical solutions. Contact sales@optofilters.com to discuss your NIR imaging requirements, request detailed specifications, or obtain pricing. Visit www.optofilters.com for complete technical resources and product information.
