BP585nm Bandpass Filter | FWHM 20nm, Thickness 1.0mm, OD6 Blocking
The BP585-20nm-1.0mm bandpass filter combines precise spectral selectivity with exceptional out-of-band rejection, delivering superior performance for demanding multi-channel fluorescence applications. This advanced bandpass filter features a 20nm full width at half maximum (FWHM) centered at 585nm with average transmission greater than 90% across the 580-590nm passband. The extraordinary blocking performance achieves average optical density greater than 6 (OD6) from 350-565nm and 602.5-800nm, with OD greater than 3 from 800-1050nm, providing exceptional channel isolation in multi-color imaging systems. The compact 1.0mm substrate thickness enables integration into space-constrained optical assemblies while maintaining excellent mechanical stability. Manufactured by OPTOStokes using advanced high precision coating technology, this filter delivers the performance required for high-end research and clinical instrumentation.
Technical Specifications
| Parameter | Specification |
|---|---|
| Center Wavelength (CWL) | 585nm ± 2nm |
| Transmission Range | 580-590nm at average T greater than 90% |
| Full Width at Half Maximum (FWHM) | 20nm ± 2nm |
| Blocking Zone 1 | 350-565nm at average OD greater than 6 (minimum OD greater than 5) |
| Blocking Zone 2 | 602.5-800nm at average OD greater than 6 (minimum OD greater than 5) |
| Blocking Zone 3 | 800-1050nm at OD greater than 3 |
| Substrate Thickness | 1.0mm |
| Surface Quality | 60/40 scratch-dig per MIL-PRF-13830B |
| Coating Type | Hard ion-assisted deposition, advanced multi-layer design |
Key Performance Features
This high OD bandpass filter achieves exceptional spectral performance optimized for multi-channel fluorescence applications. The 20nm FWHM provides an optimal balance between wavelength selectivity and light collection, efficiently capturing emissions from PE-Texas Red, mCherry, and other orange-red fluorophores while maintaining adequate separation from adjacent channels.
The extraordinary multi-zone blocking architecture delivers unprecedented rejection performance. Zone 1 (350-565nm) achieves average OD6, providing greater than 1,000,000-fold suppression of green and yellow fluorescence channels and excitation light. Zone 2 (602.5-800nm) maintains average OD6, completely eliminating far-red channel crosstalk. Zone 3 (800-1050nm) provides OD3 blocking, suppressing near-infrared detector noise and scattered light. This comprehensive rejection ensures absolutely clean signal detection in spectrally crowded multi-parameter systems.
Average transmission exceeding 90% across the 580-590nm passband maximizes signal collection from target fluorophores. The compact 1.0mm thickness reduces optical path length, minimizes focal shift, and enables high-density filter packing in automated systems. The robust hard coating ensures long-term durability and fluorescence optics performance stability.
Applications
The BP585-20nm-1.0mm filter excels in applications demanding extreme channel isolation and high contrast:
Multi-Parameter Flow Cytometry: Advanced flow cytometry panels with 15-30 parameters require exceptional spectral separation. The OD6 blocking eliminates spillover from green, yellow, and far-red channels, enabling accurate detection of PE-Texas Red, PE-Cy5, and similar tandem conjugates. Clinical diagnostics, immunophenotyping, and rare cell detection benefit from the superior channel isolation this fluorescence detection capability provides.
Multi-Color Fluorescence Microscopy: Imaging systems utilizing 4-6 simultaneous fluorescence channels require filters with deep blocking to prevent crosstalk. The BP585 filter enables clean detection of mCherry, tdTomato, and DsRed proteins while completely rejecting GFP, YFP, and Cy5 emissions. Super-resolution microscopy and live-cell imaging applications benefit from the enhanced contrast and reduced background.
High-Content Screening: Automated imaging platforms analyzing thousands of samples require reproducible, high-contrast fluorescence detection. The OD6 blocking ensures consistent performance across plate formats, while the 1.0mm thickness facilitates rapid filter wheel switching. Pharmaceutical research and drug discovery applications rely on this precision performance.
Confocal and Laser Scanning Microscopy: Point-scanning systems benefit from the extreme laser line rejection this filter provides. The OD6 blocking at typical 561nm excitation wavelengths prevents detector saturation, enabling detection of weak fluorescence signals in thick specimens and tissue sections.
Customization and Service
OPTOStokes provides comprehensive customization options. Center wavelength can be tuned across 580-590nm. FWHM can be adjusted from 15-25nm. Blocking specifications can be enhanced or extended to additional spectral regions. Geometric options include circular, square, and custom bandpass shapes. Mounting solutions range from unmounted to integrated filter sets for simplified system integration.
Quality and Availability
Every filter undergoes rigorous spectral verification across the full UV-NIR range, documenting OD performance at critical wavelengths. Comprehensive quality documentation supports customer quality systems and regulatory requirements. OPTOStokes maintains inventory for rapid delivery and offers expedited custom manufacturing.
Contact Us
The BP585-20nm-1.0mm filter exemplifies OPTOStokes' expertise in high-performance optical filters. Contact sales@optofilters.com to discuss requirements, request specifications, or obtain pricing. Visit www.optofilters.com for complete technical resources.
