Common fluorescent group spectral curves
Introduction to Fluorescence Filters
Fluorescence filters are critical components in fluorescence microscopy, enabling precise separation of excitation and emission light for clear imaging. MOVO OPTO offers high-precision optical filters optimized for superior fluorescence detection.
How Fluorophores Work
Fluorophores, or fluorescent dyes, absorb energy at specific wavelengths (e.g., UV or blue light) and emit light at longer wavelengths (e.g., green or red). Matching peak excitation and emission wavelengths with filters ensures optimal image clarity.
Components of Fluorescence Filters
Fluorescence filter sets typically include:
- Excitation Filter: Allows only the excitation wavelength to pass, often a bandpass filter.
- Emission Filter: Transmits the fluorescence signal while blocking other light, requiring high optical density (OD5+).
- Dichroic Mirror: Positioned at 45°, reflects excitation light (>90% reflectivity) and transmits emission light (>90% transmittance).
Applications and Performance
Used in PCR machines, fluorescence immunoassays, and microscopes, these filters demand high cutoff depth (OD5+) and excellent surface quality to prevent blurry images.
Why Choose MOVO OPTO?
MOVO OPTO delivers custom fluorescence filters for UV, VIS, NIR, IR, and fluorescence applications, meeting global standards. Visit www.optofilters.com for tailored solutions.
